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Ephrin B1, B2, B3 Polyclonal Antibody
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FIGURE: 1 / 4
Ephrin B1, B2, B3 Antibody (600-401-MP0S) in ICC/IF
Immunofluorescence of Rabbit Anti-Ephrin B1-B3 Antibody. (A). Neural Plate of Xenopus laevis stage 17. (B). Knockdown neural plate of Xenopus laevis stage 17. Primary Antibody: Anti-Ephrin B1-3 at 1:5000 overnight at 2-8°C. Secondary Antibody: Anti-Rabbit IgG Fluorescent Secondary. Fixative: 4% Paraformaldehyde in PBS. Permeabilization: 0.05% Triton-X100 in PBS. Nuclear Counterstain: None. Magnifica... View More
Product Details
600-401-MP0S
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Xenopus
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Anti-Ephrin B1-B3 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to C-terminal portion of human Ephrin B1, B2, B3 conjugated to Keyhole Limpet Hemocyanin (KLH).
Conjugate
Form
Liquid
Concentration
1.05 mg/mL
Amount
26.25 µg
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This affinity purified antibody is directed against human Ephrin B1, B2, B3. A BLAST analysis was used to suggest cross-reactivity with the antigen based on 100% homology with Ephrin-B1 to human, mouse, rat, and Gallus gallus; 100% homology with Ephrin-B2 to mouse; 100% homology with Ephrin-B3 to human and mouse.
Target Information
Eph receptors and ephrins exhibit complementary expression in many tissues during embryogenesis indicating that bidirectional activation of Eph receptors and ephrin-B proteins may occur at expression domain interfaces. Ephrin-B1 transduces outside-in signals through C-terminal protein interactions that effect integrin-mediated cell attachment and migration. The distribution of ephrin-B1 in the developing retina suggests that it influences retinal axon mapping along the dorsal-ventral axis and may be involved in intratectal development The transmembrane ligand ephrin-B2 and its receptor tyrosine kinase EphB4 are specifically expressed on arterial and venous endothelial cells, respectively.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CEK5 receptor ligand; CEK5-L; EFL-3; ELF-2; ELK ligand; ELK-L; EPH-related receptor transmembrane ligand ELK-L3; EPH-related receptor tyrosine kinase ligand 2; EPH-related receptor tyrosine kinase ligand 5; EPH-related receptor tyrosine kinase ligand 8; Ephrin-B1; Ephrin-B2; Ephrin-B3; HTK ligand; HTK-L; LERK-2; LERK-5; LERK-8; ligand of eph-related kinase 5; ligand of the eph-related receptor tyrosine kinases hek and elk; Stimulated by retinoic acid gene 1 protein; unnamed protein product
Gene Aliases: Cek5-L; CFND; CFNS; EFB1; EFL-3; EFL-6; EFL3; EFL6; EFNB1; EFNB2; EFNB3; ELF-2; ELF-3; Elf2; Elk-L; Elk-L3; ephrin-B2; Epl2; Epl5; Epl8; EPLG2; EPLG5; EPLG8; Htk-L; HTKL; LERK-2; LERK-5; LERK-8; LERK2; LERK5; LERK8; NLERK-1; NLERK-2; Stra1
UniProt ID: (Human) P98172, (Human) P52799, (Human) Q15768, (Mouse) P52795, (Mouse) P52800, (Mouse) O35393
Entrez Gene ID: (Human) 1947, (Human) 1948, (Human) 1949, (Mouse) 13641, (Mouse) 13642, (Mouse) 13643
neural crest cell migration
cell adhesion
cell-cell signaling
nervous system development
axon guidance
embryonic pattern specification
cell differentiation
T cell costimulation
T cell proliferation
positive regulation of T cell proliferation
ephrin receptor signaling pathway
regulation of autophagosome assembly
angiogenesis
lymph vessel development
cell migration involved in sprouting angiogenesis
positive regulation of cell proliferation
anatomical structure morphogenesis
organ morphogenesis
negative regulation of keratinocyte proliferation
negative regulation of neuron projection development
adherens junction organization
keratinocyte proliferation
viral entry into host cell
blood vessel morphogenesis
venous blood vessel morphogenesis
regulation of chemotaxis
cellular response to lipopolysaccharide
nephric duct morphogenesis
presynapse assembly
regulation of postsynaptic membrane neurotransmitter receptor levels
regulation of postsynaptic neurotransmitter receptor internalization
positive regulation of aorta morphogenesis
positive regulation of cardiac muscle cell differentiation
adult walking behavior
axon choice point recognition
negative regulation of axonogenesis
trans-synaptic signaling by trans-synaptic complex, modulating synaptic transmission
multicellular organism development
cell migration
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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