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FIGURE: 1 / 6
SMAD2 Antibody (700048RP555) in ICC/IF
Immunofluorescence analysis of SMAD2 was performed using SMAD2 Recombinant Rabbit Monoclonal Antibody (31H15L4), Alexa Fluor™ Plus 555 Antibody (Product # 700048RP555) on 70-80% confluent untreated and serum starved (overnight) TGF beta (20 ng/mL for 1 hour) treated HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.5% Triton X-100 for 15 minutes and blocked with 2% BSA for 1 hour at room temperature. The cells were stained with SMAD2 Recombinant Rabbit Monoclonal Antibody (31H15L4), Alexa Fluor™... View More
Product Details
700048RP555
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
31H15L4
Conjugate
Excitation/Emission Max
558/572 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A/G
Storage buffer
proprietary buffer, pH 6.3-7.3
Contains
0.008% Bromonitrodioxane, 0.008% Methylisothiazolone
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Wet ice
RRID
Product Specific Information
Alexa Fluor™ Plus recombinant antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning data. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor™ antibodies, providing you with better signal-to-noise for your critical experiments. These antibodies show better specificity and lot-to-lot consistency as these are recombinant antibodies, generated by cloning specific genes for the desired antibodies into an expression vector and expressed in vitro.
Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 553 nm; Emission: 568 nm; Laser: Yellow Laser
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
SMAD2 (MADH2, MAD2) regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. SMAD2 interacts with the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation into the nucleus is a central event in TGF beta signaling. Phosphorylation of threonine 8 in the calmodulin-binding region of the MH1 domain by extracellular signal regulated kinase 1 (ERK1) enhances SMAD2 transcriptional activity, which is negatively regulated by calmodulin. In response to the TGF-beta signal, SMAD2 is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. SMAD2 can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants encoding the SMAD2 protein have been observed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: hMAD-2; JV18-1; MAD; MAD homolog 2; Mad protein homolog; Mad-related protein 2; MGC22139; MGC34440; mother against DPP homolog 2; Mothers against decapentaplegic homolog 2; pSMAD2; Sma- and Mad-related protein 2; SMAD; SMAD 2; SMAD family member 2; SMAD, mothers against DPP homolog 2; Smad2Deltaexon3
Gene Aliases: hMAD-2; hSMAD2; JV18; JV18-1; MADH2; MADR2; SMAD2
UniProt ID: (Human) Q15796
Entrez Gene ID: (Human) 4087
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
DNA binding
chromatin binding
double-stranded DNA binding
transcription factor activity, sequence-specific DNA binding
transforming growth factor beta receptor binding
protein binding
transcription factor binding
phosphatase binding
transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity
ubiquitin protein ligase binding
activating transcription factor binding
type I transforming growth factor beta receptor binding
enhancer binding
protein homodimerization activity
SMAD binding
metal ion binding
protein heterodimerization activity
co-SMAD binding
I-SMAD binding
R-SMAD binding
DNA-binding transcription factor
gene-specific transcriptional regulator
negative regulation of transcription from RNA polymerase II promoter
ureteric bud development
in utero embryonic development
endoderm formation
mesoderm formation
transcription from RNA polymerase II promoter
transforming growth factor beta receptor signaling pathway
common-partner SMAD protein phosphorylation
SMAD protein complex assembly
zygotic specification of dorsal/ventral axis
gastrulation
negative regulation of cell proliferation
response to glucose
post-embryonic development
anterior/posterior pattern specification
positive regulation of epithelial to mesenchymal transition
regulation of transforming growth factor beta receptor signaling pathway
signal transduction involved in regulation of gene expression
insulin secretion
lung development
negative regulation of transforming growth factor beta receptor signaling pathway
positive regulation of BMP signaling pathway
pancreas development
primary miRNA processing
activin receptor signaling pathway
somatic stem cell population maintenance
organ growth
intracellular signal transduction
nodal signaling pathway
cell fate commitment
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
paraxial mesoderm morphogenesis
embryonic foregut morphogenesis
embryonic cranial skeleton morphogenesis
regulation of binding
palate development
pericardium development
SMAD protein signal transduction
response to cholesterol
positive regulation of nodal signaling pathway involved in determination of lateral mesoderm left/right asymmetry
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