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CD276 (B7-H3) Antibody (740074TP555) in WB
Western blot was performed using CD276 (B7-H3) Recombinant Rat Monoclonal Antibody (M3.2D7), Alexa Fluor™ Plus 555 (Product # 740074TP555, 1:100) and a ~100 kDa band corresponding to CD276 (B7-H3) was observed across cell lines and tissues tested. Whole cell extracts (60 µg lysate) of LNCaP (Lane 1), U-2 OS (Lane 2), PC-3 (Lane 3), HeLa (Lane 4), SK-OV-3 (Lane 5), A-431 (Lane 6), HT-29 (Lane 7), Hep G2 (Lane 8) and Jurkat (Lane 9) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were th... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
740074TP555
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rat
/ IgG2a
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
M3.2D7
Conjugate
Alexa Fluor™ Plus 555
Alexa Fluor™ Plus 555
Alexa Fluor™ Plus 555
Excitation/Emission Max
558/572 nm
View spectra
Form
Liquid
Concentration
1.0 mg/mL
Amount
50 µg
Purification
Affinity chromatography
Storage buffer
proprietary buffer, pH 6.8
Contains
0.008% Bromonitrodioxane, 0.008% Methylisothiazolone
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Wet ice
RRID
Product Specific Information
Alexa Fluor™ Plus recombinant antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning data. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor™ antibodies, providing you with better signal-to-noise for your critical experiments. These antibodies show better specificity and lot-to-lot consistency as these are recombinant antibodies, generated by cloning specific genes for the desired antibodies into an expression vector and expressed in vitro.
Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.
Applications Tested: This M3.2D7 antibody has been tested by flow cytometry and western blot. This may be used for flow cytometry at 2 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 553 nm; Emission: 568 nm; Laser: Yellow Laser
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD276 is a member of the B7 family of co-stimulatory molecules also known as B7-H3. CD276 is a type I transmembrane protein that induces the proliferation of CD4+ and CD8+ T cells, enhances the generation of cytotoxic T cells and selectively stimulates the production of interferon gamma. Expression of CD276 can be induced on dendritic cells and monocytes by inflammatory cytokines, and is also widely expressed in peripheral tissues including the heart, kidney, testis and colon. In humans, CD276 exists as two isoforms which result from gene duplication and differential splicing. CD276 is reported to have therapeutic potential for the regulation of cell-mediated immune responses to cancer, particularly in conjunction with anti-angiogenic therapy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: B7 homolog 3; costimulatory molecule; Ig protein; sB7 H3; sB7H3; sCD276; soluble B7 H3; soluble B7H3; soluble CD276; unnamed protein product
Gene Aliases: 4Ig-B7-H3; 6030411F23Rik; B7-H3; B7H3; B7RP-2
UniProt ID: (Human) Q5ZPR3, (Mouse) Q8VE98
Entrez Gene ID: (Human) 80381, (Mouse) 102657
regulation of cytokine production
positive regulation of cytokine production
positive regulation of bone mineralization
regulation of interferon-gamma production
negative regulation of interferon-gamma production
negative regulation of interleukin-2 production
positive regulation of interferon-gamma production
positive regulation of interleukin-2 production
positive regulation of T cell proliferation
T cell activation
negative regulation of T cell proliferation
positive regulation of osteoblast differentiation
negative regulation of inflammatory response
regulation of immune response
T cell receptor signaling pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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