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CD276 (B7-H3) Antibody (740074T) in IHC (P)
Immunohistochemical analysis of CD276 (B7-H3) was performed using formalin-fixed paraffin-embedded mouse testis tissue sections. To expose the target protein, heat-induced epitope retrieval was performed on de-paraffinized sections using eBioscience™ IHC Antigen Retrieval Solution - High pH (10X) (Product # 00-4956-58) diluted to 1X solution in water in a decloaking chamber at 110 degree Celsius for 15 minu... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
740074T
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rat
/ IgG2a
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
M3.2D7
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1.0 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2-7.4
Contains
0.09% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
Product Specific Information
Recombinant rat monoclonal antibodies are produced using in vitro expression systems. Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include: better specificity, lot-to-lot consistency, and broader immunoreactivity to diverse targets due to a larger rabbit immune repertoire.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Target Information
CD276 is a member of the B7 family of co-stimulatory molecules also known as B7-H3. CD276 is a type I transmembrane protein that induces the proliferation of CD4+ and CD8+ T cells, enhances the generation of cytotoxic T cells and selectively stimulates the production of interferon gamma. Expression of CD276 can be induced on dendritic cells and monocytes by inflammatory cytokines, and is also widely expressed in peripheral tissues including the heart, kidney, testis and colon. In humans, CD276 exists as two isoforms which result from gene duplication and differential splicing. CD276 is reported to have therapeutic potential for the regulation of cell-mediated immune responses to cancer, particularly in conjunction with anti-angiogenic therapy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: B7 homolog 3; costimulatory molecule; Ig protein; sB7 H3; sB7H3; sCD276; soluble B7 H3; soluble B7H3; soluble CD276; unnamed protein product
Gene Aliases: 4Ig-B7-H3; 6030411F23Rik; B7-H3; B7H3; B7RP-2
UniProt ID: (Human) Q5ZPR3, (Mouse) Q8VE98
Entrez Gene ID: (Human) 80381, (Mouse) 102657
regulation of cytokine production
positive regulation of cytokine production
positive regulation of bone mineralization
regulation of interferon-gamma production
negative regulation of interferon-gamma production
negative regulation of interleukin-2 production
positive regulation of interferon-gamma production
positive regulation of interleukin-2 production
positive regulation of T cell proliferation
T cell activation
negative regulation of T cell proliferation
positive regulation of osteoblast differentiation
negative regulation of inflammatory response
regulation of immune response
T cell receptor signaling pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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