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Bethyl Laboratories
Ets-1 Polyclonal Antibody
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FIGURE: 1 / 1
Ets-1 Antibody (A303-501A-T) in WB
Detection of human Ets-1 by western blot and immunoprecipitation. Samples: Whole cell lysate from Jurkat (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg) and HeLa (H; 50 µg) cells. Antibodies: Affinity purified rabbit anti-Ets-1 antibody (Product # A303-501A) used for WB at 0.1 µg/mL (A) and 1 µg/mL (B) and used for IP at 6 µg/mg lysate. Ets-1 was also immunoprecipitated by rabbit anti-Ets-1 antibody A303-500A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) an... View More
Product Details
A303-501A-T
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 391 and 441 of human v-ets Erythroblastosis Virus E26 Oncogene Homolog 1.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
10 µg
Purification
Antigen affinity chromatography
Storage buffer
tris citrate/phosphate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Based on 100% sequence identity, this antibody is predicted to react with Rat and Chicken
Target Information
ETS1 (c-Ets-1; v-ets erythroblastosis virus E26 oncogene homlog 1 (avian); ETS-1; EWSR2; p54), a 441 A. A protein, is a member of DNA-binding ETS protein family. It is a multifunctional protein consisting of an N-terminal pointed domain, a central transactivation domain and 2 auto inhibitory domains flanked on both sides of conserved C-terminal Ets domain. It interacts with factors including PEBP2alphaA, p300/CBP and mediates transactivation of Osteopontin, Presenilin-1, Stromelysin and Collagenase. It is activated by Ras/MAPK signaling and its expression is up-regulated in resting T-cells. Its activity is modulated by various factors including AP-1, Sp-1, c-myb and MafB. Sp100 activates ETS1 in nuclear bodies. It interacts with EAP1/Daxx and causes repression of MMP1 and BCL2 transactivation. It interacts with STAT6 and modulates Socs-1 expression induced by IL-4. It interacts with GATA3 and positively regulates Tax-1 dependent IL-5 expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Avian erythroblastosis virus E26 (v-ets) oncogene homolog-1; ets protein; ets-1 protein; Ets-1Delta(III-VI); ETS1; FLJ10768; p54; Protein C-ets-1; unnamed protein product; v-ets avian erythroblastosis virus E2 oncogene homolog 1; v-ets avian erythroblastosis virus E26 oncogene homolog 1
Gene Aliases: c-ets-1; ETS-1; ETS1; EWSR2; p54
UniProt ID: (Human) P14921
Entrez Gene ID: (Human) 2113
transcription regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcription corepressor binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
nucleic acid binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
identical protein binding
sequence-specific DNA binding
RNA polymerase II sequence-specific DNA binding transcription factor binding
sequence-specific double-stranded DNA binding
immune system process
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
immune response
negative regulation of cell proliferation
positive regulation of endothelial cell migration
positive regulation of gene expression
cell differentiation
PML body organization
regulation of apoptotic process
positive regulation of blood vessel endothelial cell migration
positive regulation of erythrocyte differentiation
regulation of angiogenesis
positive regulation of angiogenesis
negative regulation of cell cycle
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
response to antibiotic
cell motility
positive regulation of inflammatory response
positive regulation of pri-miRNA transcription from RNA polymerase II promoter
positive regulation of leukocyte adhesion to vascular endothelial cell
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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