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6x His, His-Tag Monoclonal Antibody (1B7G5), APC
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Product Details
APC-66005
Product Specifications
Species Reactivity
Tag
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
1B7G5
Immunogen
Peptide
Conjugate
Excitation/Emission Max
651/660 nm
View spectra
Form
Liquid
Concentration
1000 µg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS with 0.5% BSA
Contains
0.1% sodium azide
Storage conditions
-20°C, store in dark
Shipping conditions
Wet ice
Target Information
The 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH). The His tag is commonly expressed as a tag at either N-or C-terminal regions of recombinant proteins to allow isolation or purification by immobilized metal affinity chromatography. Epitope tagging is a technique in which a known epitope is fused to a recombinant protein using genetic engineering. By choosing a particular epitope and recombinant protein combination, epitope tagging makes it possible to detect proteins for which no antibody is available. His epitope tagged proteins that contain a stretch of histidine residues at the carboxyl or amino terminus are used for purification of recombinant proteins by means of metal chelate chromatography due to metal binding properties of histidine clusters on His tagged proteins. For example, the affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent. His tag-specific antibodies are used to facilitate detection or coimmunopreciptation of tagged proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 6His tag; 6X His; His Tag; Histidine Tag
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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