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His-Tag Monoclonal Antibody (1B7G5), HRP
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Product Details
HRP-66005
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Tag
Published species
Tag
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
1B7G5
Immunogen
6xHis peptide
Conjugate
Form
Liquid
Concentration
0.4 mg/mL
Amount
40 µg
Purification
Protein G
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.5% BSA
Contains
0.02% thimerosal, 0.05% ProClin 300
Storage conditions
-20°C, store in dark
Shipping conditions
Wet ice (domestic); Dry ice (international)
Product Specific Information
This antibody is conjugated with HRP. Note: Do not add azide (Sodium Azide or Smite) into the dilution buffer. azide is the HRP inhibitor which decreases the enzymatic activity of HRP.
Target Information
The 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH). The His tag is commonly expressed as a tag at either N-or C-terminal regions of recombinant proteins to allow isolation or purification by immobilized metal affinity chromatography. Epitope tagging is a technique in which a known epitope is fused to a recombinant protein using genetic engineering. By choosing a particular epitope and recombinant protein combination, epitope tagging makes it possible to detect proteins for which no antibody is available. His epitope tagged proteins that contain a stretch of histidine residues at the carboxyl or amino terminus are used for purification of recombinant proteins by means of metal chelate chromatography due to metal binding properties of histidine clusters on His tagged proteins. For example, the affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent. His tag-specific antibodies are used to facilitate detection or coimmunopreciptation of tagged proteins.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 6His tag; 6X His; His Tag; Histidine Tag
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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