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Invitrogen

Bcl-2 Monoclonal Antibody (Bcl-2/100), FITC, eBioscience™

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Product Details

BMS1028FI

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

Assay-Dependent
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

Bcl-2/100

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra spectra

Form

Liquid

Concentration

0.1 mg/mL

Amount

100 µg

Purification

Affinity chromatography

Storage buffer

TBS, pH 7.2-7.4, with 1% BSA

Contains

0.02% sodium azide

Storage conditions

-20°C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10596978

Product Specific Information

Description: Bcl-2 is a key regulator of apoptosis and the founding member of Bcl-2 family of apoptosis regulatory protiens. The anti-apoptotic functions of Bcl-2 is achieved by activation or inactivation of an inner mitochondrial permeability transition pore, which is involved in the regulation of matrix Ca2+, pH, and voltage.

Applications Tested: Flow Cytometry.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser

Target Information

BCL-2 is a key regulator of apoptosis that functions to either inhibit or promote cell death. The BCL-2 family members are also characterized by dimerizing to further modulate apoptosis. Bag1, for example, has been found to form a heterodimer with BCL-2 resulting in the enhancement of the anti-apoptotic effect of BCL-2. Bax and Bak have been shown to play a critical role in cytochrome c release from mitochondria and thus initiate apoptosis. Bax exerts a pro-apoptotic rather than an anti-apoptotic effect on cells. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. In most follicular lymphomas, neoplastic germinal centers express high levels of BCL-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Two transcript variants of BCL-2, produced by alternate splicing, differ in their C-terminal ends. The overexpression of BCL-2 has been linked to human cancers such as B-cell lymphoma and prostate cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

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Bioinformatics

Protein Aliases: B-cell CLL/lymphoma 2; Bcl 2; bcl-2 protein; bcl2-alpha protein; bcl2-beta protein; bcl2-Ig fusion gene; OTTHUMP00000163680; protein phosphatase 1, regulatory subunit 50

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Gene Aliases: Bcl-2; PPP1R50

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UniProt ID: (Human) P10415

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Entrez Gene ID: (Human) 596

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Function(s)
protease binding protein binding channel activity channel inhibitor activity protein phosphatase binding ubiquitin protein ligase binding identical protein binding sequence-specific DNA binding protein heterodimerization activity BH3 domain binding protein phosphatase 2A binding binding, bridging DNA-binding transcription factor binding
Process(es)
protein polyubiquitination release of cytochrome c from mitochondria apoptotic process humoral immune response cellular response to DNA damage stimulus female pregnancy positive regulation of cell proliferation extrinsic apoptotic signaling pathway via death domain receptors intrinsic apoptotic signaling pathway in response to DNA damage response to radiation response to xenobiotic stimulus response to toxic substance response to iron ion regulation of autophagy negative regulation of autophagy programmed cell death positive regulation of cell growth positive regulation of B cell proliferation hair follicle morphogenesis endoplasmic reticulum calcium ion homeostasis response to cytokine response to nicotine B cell proliferation regulation of apoptotic process positive regulation of apoptotic process negative regulation of apoptotic process pigmentation regulation of neuron apoptotic process negative regulation of neuron apoptotic process regulation of viral genome replication regulation of mitochondrial membrane permeability B cell receptor signaling pathway neuron apoptotic process defense response to virus regulation of mitochondrial membrane potential negative regulation of mitochondrial depolarization regulation of calcium ion transport transmembrane transport intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress extrinsic apoptotic signaling pathway in absence of ligand negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator regulation of intracellular signal transduction negative regulation of anoikis regulation of apoptotic signaling pathway negative regulation of apoptotic signaling pathway negative regulation of extrinsic apoptotic signaling pathway in absence of ligand negative regulation of intrinsic apoptotic signaling pathway
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