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Identifyn
gamma-H2AX SRM Recombinant Rabbit Monoclonal Antibody (BLR014M), Alexa Fluor™ 594, Identifyn™
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FIGURE: 1 / 8
gamma-H2AX SRM Antibody (DC000019) in ICC/IF
IdentifynTM detailed protocols can be found below under Documents & Downloads. Western Blot: HEK 293 cells were damaged with 200 µM etoposide for 24 hours. Cells were then homogenized using an ultrasonication probe and RIPA lysis buffer and then centrifuged to pellet down the cell debris. The lysate supernatant (100 µg) was mixed with NuPAGE LDS Sample Buffer (4X), heated at 80 °C for 10 min, and subjected ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
DC000019
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
ExpiCHO
Class
Recombinant Monoclonal
Type
Antibody
Clone
BLR014M
Immunogen
Proprietary peptide specific to serine 139 phosphorylation.
Conjugate
Alexa Fluor™ 594
Alexa Fluor™ 594
Alexa Fluor™ 594
Excitation/Emission Max
590/618 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
50 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 10% glycerol
Contains
0.02% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Identifyn™ Primary and Secondary antibodies are extensively validated in Immunofluorescence assays by traditional as well as high resolution acquisition like Widefield SIM, Confocal, Airyscan and STORM Microscopy.
Recommended dilution for traditional microscopy:
Widefield - 1:1,000
Widefield SIM - 1:1,000
Confocal - 1:1,000
Recommended dilution for Super Resolution microscopy:
SIM (Structured Illumination Microscopy) - 1:1,000
Single Molecule STORM (Stochastic Optical Reconstruction Microscopy) - 1:200
Detailed protocols can be found below under Methods & Protocols.
Target Information
H2AX is a variant of the H2A protein family and is a component of the histone octomer in nucleosomes. H2AX is phosphorylated by kinases such as ataxia telangiectasia mutated (ATM) and ATM-Rad3-related (ATR) in the PI3K pathway. Gamma-H2AX is the first step in recruiting and localizing DNA repair proteins. DSBs can be induced by mechanisms such as ionizing radiation or cytotoxic agents and subsequently, gamma-H2AX foci quickly form. These foci represent the DSBs in a 1:1 manner and can be used as a biomarker for damage. The visualization of gamma-H2AX by can allow the assessment of DNA damage, related DNA damage proteins and DNA repair. Gamma-H2AX has applications in the detection of genomic damage caused by cytotoxic chemical agents and environmental and physical damage.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: gamma H2A X; gamma H2A.X; gamma-H2A.X; gh2ax; H2a/x; Histone H2A.X; Histone H2AX
Gene Aliases: H2AFX; H2AX
Entrez Gene ID: (Human) 3014
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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