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|Tested species reactivity||Mouse|
|Published species reactivity||Mouse|
|Host / Isotype||Rat / IgG2a|
|Immunogen||Recombinant mouse IL-2|
|Purification||Caprylic acid precipitation|
|Storage buffer||PBS with 4% BSA|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MM102B targets IL-2 in ELISA, and WB applications and shows reactivity with mouse samples.
The MM102B immunogen is recombinant mouse IL-2.
MM102B detects IL-2 which has a predicted molecular weight of approximately 17 kDa.
The MM102B mouse IL2 antibody (biotinylated conjugate of clone 1A12) has successfully been paired as the detection antibody in a sandwich ELISA with capture antibody MM101 (clone 5H4). Typical dilutions for sandwich ELISA include 1 µg/ml for coating and 0.125 - 0.25 µg/ml for detection.
Biotinylated antibody MM102B (clone 1A12) and antibody MM101 (clone 5H4) have successfully been used in combination with recombinant mouse IL2 protein SMIL2 in ELISA applications.
This antibody is produced by injecting Rat IgG secreting hybridoma cells into the peritoneum of mice. The resulting ascites is collected from the mice and the antibody is purified.
The protein encoded by this gene is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
An atypical CD8 T-cell response to Chlamydia muridarum genital tract infections includes T cells that produce interleukin-13.
MM102B was used in ELISA to study the mechanisms underlying CD8 T-cell-mediated pathology during genital tract Chlamydia infections and the significance of IL-13-producing cells
|Johnson RM,Kerr MS,Slaven JE||Immunology (142:248)||2014|
PmpG303-311, a protective vaccine epitope that elicits persistent cellular immune responses in Chlamydia muridarum-immune mice.
MM102B was used in ELISA to study a protective vaccine epitope that elicits persistent cellular immune responses in Chlamydia muridarum-immune mice
|Johnson RM,Yu H,Kerr MS,Slaven JE,Karunakaran KP,Brunham RC||Infection and immunity (80:2204)||2012|
Plac8-dependent and inducible NO synthase-dependent mechanisms clear Chlamydia muridarum infections from the genital tract.
MM102B was used in ELISA to investigate Plac8-dependent and iNOS-dependent mechanisms of clearing Chlamydia muridarum genital tract infections
|Johnson RM,Kerr MS,Slaven JE||Journal of immunology (Baltimore, Md. : 1950) (188:1896)||2012|
Toxoplasma gondii glycosylphosphatidylinositols up-regulate major histocompatibility complex (MHC) molecule expression on primary murine macrophages.
MM102B was used in ELISA to study the effect of T. gondii glycosylphosphatidylinositols on MHC expression in macrophages
|Debierre-Grockiego F,Molitor N,Schwarz RT,Lüder CG||Innate immunity (15:25)||2009|
Up-regulation of IL-4 production by the activated cAMP/cAMP-dependent protein kinase (protein kinase A) pathway in CD3/CD28-stimulated naive T cells.
MM102B was used in ELISA to investigate the role of cAMP/PKA pathway for the modulation of interleukin 4 expression in T cells
|Tokoyoda K,Tsujikawa K,Matsushita H,Ono Y,Hayashi T,Harada Y,Abe R,Kubo M,Yamamoto H||International immunology (16:643)||2004|
Commitment of individual Th1-like lymphocytes to expression of IFN-gamma versus IL-4 and IL-10: selective induction of IL-10 by sequential stimulation of naive Th cells with IL-12 and IL-4.
MM102B was used in ELISA to study the mechanism for T cell differentiation
|Assenmacher M,Löhning M,Scheffold A,Richter A,Miltenyi S,Schmitz J,Radbruch A||Journal of immunology (Baltimore, Md. : 1950) (161:2825)||1998|
Th1 development of naive CD4+ T cells is inhibited by co-activation with anti-CD4 monoclonal antibodies.
MM102B was used in ELISA to investigate the effect of CD4 antibodies on Th1 differentiation
|Goedert S,Germann T,Hoehn P,Koelsch S,Palm N,Rüde E,Schmitt E||Journal of immunology (Baltimore, Md. : 1950) (157:566)||1996|
Corticosteroids enhance the capacity of macrophages to induce Th2 cytokine synthesis in CD4+ lymphocytes by inhibiting IL-12 production.
MM102B was used in blocking/activating experiment to investigate the changes of IL-12 expression modulated by corticosteroids in CD4+ T cells
|DeKruyff RH,Fang Y,Umetsu DT||Journal of immunology (Baltimore, Md. : 1950) (160:2231)||1998|
interleukin-2; T-cell growth factor; TCGF