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Invitrogen
c-Kit Monoclonal Antibody (104D2)
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Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA1-10072
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Human,
Non-human primate
Published species
Human
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
104D2
Immunogen
MOLM-1 megakaryocytic cells
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
15mM sodium azide
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody detects extracellular part of CD117 / c-Kit protooncogen.
Target Information
KIT (c-KIT) is a proto-oncogene and a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). KIT was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. KIT together with its ligand regulates growth and activation of a variety of hemopoietic and non-hemopoietic cells. Mutations in KIT are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism. Recently, deregulation of the KIT receptor TK by the prevalent activation loop mutation D816V has served as a focal point in therapeutic strategies aimed at curbing neoplastic mast cell growth. c-Kit is expressed in hematopoietic stem cells, germ cells, mast cells and gastrointestinal tract cajal cells. Upon binding of its ligand stem cell factor (SCF), c-kit dimerizes, resulting in receptor activation and autophosphorylation of various tyrosine residues including tyrosine 703 located on the cytoplasmic domain of the receptor. This modification allows docking of Grb2 and activation of the Ras/ERK signaling pathway. SCF/c-kit can activate multiple downstream signaling pathways including PI3K, PLC-gamma and JAK/STAT. c-kit receptor activation is essential for hematopoiesis, stem cell maintenance and gametogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: c-Kit protooncogene; c-kit receptor; ckit; mast cell growth factor receptor; p145 c-kit; piebald trait protein; protein kinase; proto-oncogene c-Kit; proto-oncogene tyrosine-protein kinase Kit; SCFR; tyrosine-protein kinase Kit; unnamed protein product; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein
Gene Aliases: C-Kit; CD117; MASTC; PBT; SCFR
UniProt ID: (Human) P10721
Entrez Gene ID: (Bovine) 280832, (Human) 3815
transmembrane receptor protein tyrosine kinase activity
ATP binding
cytokine binding
metal ion binding
protease binding
protein kinase activity
protein tyrosine kinase activity
stem cell factor receptor activity
protein binding
growth factor binding
SH2 domain binding
protein homodimerization activity
transmembrane signal receptor
activation of MAPK activity
ovarian follicle development
immature B cell differentiation
dendritic cell cytokine production
inflammatory response
spermatogenesis
regulation of cell shape
peptidyl-tyrosine phosphorylation
cytokine-mediated signaling pathway
lamellipodium assembly
T cell differentiation
erythrocyte differentiation
melanocyte differentiation
actin cytoskeleton reorganization
mast cell cytokine production
embryonic hemopoiesis
megakaryocyte development
Fc receptor signaling pathway
Kit signaling pathway
erythropoietin-mediated signaling pathway
mast cell degranulation
pigmentation
digestive tract development
stem cell differentiation
detection of mechanical stimulus involved in sensory perception of sound
cell chemotaxis
mast cell differentiation
melanocyte migration
melanocyte adhesion
hematopoietic progenitor cell differentiation
mast cell chemotaxis
positive regulation of cytokine production involved in immune response
positive regulation of dendritic cell cytokine production
chemotaxis
signal transduction
transmembrane receptor protein tyrosine kinase signaling pathway
positive regulation of cell proliferation
germ cell migration
gonad development
visual learning
male gonad development
cell migration
stem cell population maintenance
actin cytoskeleton organization
hemopoiesis
B cell differentiation
positive regulation of cell migration
positive regulation of pseudopodium assembly
positive regulation of mast cell cytokine production
somatic stem cell population maintenance
regulation of cell proliferation
positive regulation of tyrosine phosphorylation of STAT protein
positive regulation of MAPK cascade
tongue development
leukocyte activation
positive regulation of Notch signaling pathway
positive regulation of JAK-STAT cascade
response to cadmium ion
protein autophosphorylation
positive regulation of long-term neuronal synaptic plasticity
epithelial cell proliferation
regulation of developmental process
positive regulation of protein kinase B signaling
mast cell proliferation
positive regulation of pyloric antrum smooth muscle contraction
positive regulation of colon smooth muscle contraction
positive regulation of small intestine smooth muscle contraction
positive regulation of vascular smooth muscle cell differentiation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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