Stem cell research & therapy 2022 - Representative images of immunohistochemically stained murine gastrocnemius muscle for CD31 (green) and CD11b (red) ( n = 6). A DAPI was used to visualize nuclei staining (blue). B Quantification of CD11b- and C CD31-stained area of histological sections of muscle tissue derived from mice in the MSCs and BDACs treatment and vehicle groups

Product Image Gallery

CD11b Antibody — Figure 5 OG inhibits tumor growth, M2-type macrophages, and serum HSP90alpha levels in Panc 02 + EndoMT tumor model. ( A ) Measurement of the superficial tumor volumes from the C57BL/6 mice subcutaneously inoculated with Panc 02 + EndoMT cell grafts and treated with OG or control 40% PEG400 (CTRL). The measurement was started on Day 13 post-inoculation and continued every 3 days using Vernier caliper with the formula 1/2 x length x width 2 . ( B ) Tumors removed from the mice described in (A) on Day 32 post-inoculation. OG treatment significantly inhibited the tumor growth of Panc 02 + EndoMT cell grafts. ( C ) Representative H&E-stained tumor tissue sections of control and OG-treated mice. OG treatment did not obviously cause loosened cell arrangement and decreased chromatin basophilia which were observed in the Panc 02 tumor model. ( D ) Immunohistochemical staining of CK18 and alpha-SMA from the tumor tissues of control or OG-treated mice. OG treatment did not significantly change the CK18 + and alpha-SMA + cell levels of tumor tissues. ( E ) Immunohistochemical staining of CD11b + , F4/80 + , and CD163 + cells from the tumor tissues of control or OG-treated mice. ( F ) Quantitation of the levels of CD11b + , F4/80 + , and CD163 + cells from the tumor tissues of control or OG-treated mice. OG treatment caused a significant increase of F4/80 + macrophages but decreases of CD11b + myeloid cells and CD163 + macrophages in the tumor tissues. ( G ) Serum HSP90alpha levels of th {TM}

Immunogen image

Immunogen Image CD11b Monoclonal Antibody (M1/70.15)

Product Details

MA1-80091

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 7 publications 7 publications

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

Assay-dependent

View 2 publications 2 publications

Flow Cytometry (Flow)

1:50

View 32 publications 32 publications

Immunoprecipitation (IP)

Assay-dependent

Miscellaneous PubMed (Misc)

View 36 publications 36 publications

Product Specifications

Species Reactivity

Human, Mouse, Rabbit

Published species

Human, Mouse, Rabbit

Host/Isotype

Rat / IgG2b

Class

Monoclonal

Type

Antibody

Clone

M1/70.15

Immunogen

T cell enriched splenocytes from B10 mice.

3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

100 µg

Purification

Protein G

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_927467

Product Specific Information

This clone has been reported to block iC3b binding to its receptor.

PLP fixation is recommended for optimal tissue staining results.

Rat anti Mouse CD11b antibody, clone M1/70.15 recognizes the murine CD11b cell surface antigen also known as the alpha M integrin chain or MAC-1, a differentiation antigen expressed by granulocytes, monocytes, NK cells and tissue macrophages.

Rat anti Mouse CD11b antibody, clone M1/70.15 recognizes the murine CD11b cell surface antigen also known as the alpha M integrin chain or MAC-1, a differentiation antigen expressed by granulocytes, monocytes, NK cells and tissue macrophages.

Target Information

CD11b (integrin alpha-M, ITGAM, integrin alpha-X, ITGAX) is a 165 kDa adhesion molecule that associates non-covalently with integrin beta-2 (CD18). The CD11b/CD18 heterodimeric complex is also known as integrin alpha-M beta-2, Mac-1, and CR3 (complement receptor 3). CD11b is expressed on the surface of monocytes/macrophages, granulocytes, activated lymphocytes, a subset of NK cells, a subset of dendritic cells, and microglia in the brain. CD11b/CD18 functions as the receptor for ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X -these adhesions are critical for cell-cell and cell-matrix interactions. CD11b is expressed on 8% of spleen cells, 44% of bone marrow cells, and less than 1% of thymocytes, and is commonly used as a microglial marker in nervous tissue. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Further, peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages. Diseases associated with CD11b dysfunction include systemic lupus erythematosus 6 and ITGAM-related susceptibility to systemic lupus erythematosus.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: antigen CD11b (p170); CD11 antigen-like family member B; CD11b; CD11B (p170); cell surface glycoprotein MAC-1 alpha subunit; Cell surface glycoprotein MAC-1 subunit alpha; complement component 3 receptor 3 subunit; complement component receptor 3 alpha-a; complement receptor type 3; CR-3 alpha chain; glycoprotein Mac-1; Integrin alpha-M; integrin, alpha M (complement component 3 receptor 3 subunit); leukocyte adhesion glycoprotein precursor; Leukocyte adhesion receptor MO1; Mac-1 alpha; Mac-1 alpha (Mac1A); macrophage antigen alpha; macrophage antigen alpha polypeptide; macrophage-1 antigen alpha subunit; MGC117044; Neutrophil adherence receptor; neutrophil adherence receptor alpha-M subunit; unnamed protein product

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Gene Aliases: CD11B; CD11b/CD18; CR3; CR3A; F730045J24Rik; ITGAM; Ly-40; MAC-1; Mac-1a; MAC1; MAC1A; MO1A; SLEB6

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UniProt ID: (Human) Q4VAK1

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Entrez Gene ID: (Human) 3684, (Mouse) 16409, (Rabbit) 100351865

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Function(s)

beta-amyloid binding complement component C3b binding integrin binding protein binding heat shock protein binding signaling receptor activity cargo receptor activity macromolecular complex binding metal ion binding opsonin binding glycoprotein binding heparin binding heparan sulfate proteoglycan binding protein heterodimerization activity integrin

Process(es)

microglial cell activation immune system process complement receptor mediated signaling pathway response to ischemia receptor-mediated endocytosis phagocytosis, engulfment cell adhesion cell-matrix adhesion integrin-mediated signaling pathway response to mechanical stimulus positive regulation of metabolic process ectodermal cell differentiation forebrain development response to estradiol positive regulation of superoxide anion generation heterotypic cell-cell adhesion positive regulation of neutrophil degranulation innate immune response negative regulation of dopamine metabolic process positive regulation of protein targeting to membrane beta-amyloid clearance cell-cell adhesion cell-cell adhesion via plasma-membrane adhesion molecules response to Gram-positive bacterium complement-mediated synapse pruning vertebrate eye-specific patterning positive regulation of microglial cell mediated cytotoxicity response to curcumin leukocyte migration involved in inflammatory response leukocyte cell-cell adhesion microglia development single organismal cell-cell adhesion neutrophil chemotaxis cellular extravasation activated T cell proliferation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD11b Monoclonal Antibody (M1/70.15)

CD11b Antibody (MA1-80091) in IHC

Product Details

MA1-80091

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Amount

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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