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Invitrogen
CD11b Monoclonal Antibody (M1/70.15), PE
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Product Details
MA1-80486
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rabbit
Published species
Mouse
Host/Isotype
Rat
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
M1/70.15
Immunogen
T cells enriched splenocytes from B10 mice.
Conjugate
PE
PE
PE
Excitation/Emission Max
565/576 nm
View spectra
Form
Lyophilized
Concentration
Conc. Not Determined
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 1% BSA, 5% sucrose
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 1 mL of distilled water. After reconstitution, store product undiluted at 4°C in the dark.
For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.
Rat anti Mouse CD11b antibody, clone M1/70.15 recognizes the murine CD11b cell surface antigen also known as the alpha M integrin chain or MAC-1, a differentiation antigen expressed by granulocytes, monocytes, NK cells and tissue macrophages.
Target Information
CD11b (integrin alpha-M, ITGAM, integrin alpha-X, ITGAX) is a 165 kDa adhesion molecule that associates non-covalently with integrin beta-2 (CD18). The CD11b/CD18 heterodimeric complex is also known as integrin alpha-M beta-2, Mac-1, and CR3 (complement receptor 3). CD11b is expressed on the surface of monocytes/macrophages, granulocytes, activated lymphocytes, a subset of NK cells, a subset of dendritic cells, and microglia in the brain. CD11b/CD18 functions as the receptor for ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X -these adhesions are critical for cell-cell and cell-matrix interactions. CD11b is expressed on 8% of spleen cells, 44% of bone marrow cells, and less than 1% of thymocytes, and is commonly used as a microglial marker in nervous tissue. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Further, peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages. Diseases associated with CD11b dysfunction include systemic lupus erythematosus 6 and ITGAM-related susceptibility to systemic lupus erythematosus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: antigen CD11b (p170); CD11 antigen-like family member B; CD11B (p170); cell surface glycoprotein MAC-1 alpha subunit; cell surface glycoprotein MAC-1 subunit alpha; complement component 3 receptor 3 subunit; complement receptor type 3; CR-3 alpha chain; glycoprotein Mac-1; human neutrophil antigen 4; integrin, alpha M (complement component 3 receptor 3 subunit); leukocyte adhesion glycoprotein precursor; leukocyte adhesion receptor MO1; Mac-1 alpha; Mac-1 alpha (Mac1A); macrophage antigen alpha; macrophage antigen alpha polypeptide; macrophage-1 antigen alpha subunit; MGC117044; neutrophil adherence receptor alpha-M subunit; unnamed protein product
Gene Aliases: CD11B; CD11b/CD18; CR3; CR3A; F730045J24Rik; HNA-4; ITGAM; Ly-40; MAC-1; Mac-1a; MAC1; MAC1A; MO1A; SLEB6
UniProt ID: (Human) P11215
Entrez Gene ID: (Human) 3684, (Mouse) 16409, (Rabbit) 100351865
beta-amyloid binding
complement component C3b binding
obsolete cell adhesion receptor activity
integrin binding
protein binding
heat shock protein binding
signaling receptor activity
cargo receptor activity
macromolecular complex binding
opsonin binding
heparin binding
heparan sulfate proteoglycan binding
integrin
microglial cell activation
response to amphetamine
complement receptor mediated signaling pathway
response to ischemia
receptor-mediated endocytosis
phagocytosis, engulfment
cell adhesion
cell-matrix adhesion
integrin-mediated signaling pathway
response to mechanical stimulus
ectodermal cell differentiation
forebrain development
response to estradiol
positive regulation of superoxide anion generation
cell-cell adhesion mediated by integrin
heterotypic cell-cell adhesion
positive regulation of neutrophil degranulation
negative regulation of dopamine metabolic process
leukocyte adhesion to vascular endothelial cell
positive regulation of protein targeting to membrane
beta-amyloid clearance
cell-cell adhesion
response to Gram-positive bacterium
complement-mediated synapse pruning
vertebrate eye-specific patterning
positive regulation of microglial cell mediated cytotoxicity
response to curcumin
neutrophil apoptotic process
leukocyte migration involved in inflammatory response
phagocytosis
leukocyte cell-cell adhesion
microglia development
neutrophil chemotaxis
cellular extravasation
activated T cell proliferation
positive regulation of mast cell differentiation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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