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Invitrogen
Pan SHANK Monoclonal Antibody (N23b/49)
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FIGURE: 1 / 4
Pan SHANK Antibody (MA5-27633) in ICC/IF
Immunofluorescent analysis of Pan SHANK in human neuroblastoma cell line (SK-N-BE). Sample was fixed with 4% formaldehyde (15 min at RT), incubated with Pan SHANK monoclonal antibody (Product # MA5-27633) using a dilution of 1:100 (1 hour at RT), and followed by Goat Anti-Mouse 488, Phalloidin Texas Red and DAPI secondary antibody at a dilution of 1:200, 1:1000 (60 min at RT) and 1:5000 (5 min at RT). Image... View More
Product Details
MA5-27633
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
N23b/49
Immunogen
Fusion protein amino acids 84-309 of rat Shank2
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
1 µg/mL of MA5-27633 was sufficient for detection of Shank1-4 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 160kDa. Recognizes Shank1, 2 and 3.
Target Information
Shank proteins make up a family of scaffold proteins identified through their interaction with a variety of membrane and cytoplasmic proteins. Shank proteins at postsynaptic sites of excitatory synapses play roles in signal transmission into the postsynaptic neuron. Studies suggest that Shank2 is expressed in the neurons of the developing retina, and could play a role in the neuronal differentiation of the developing retina. Other recent studies suggest that the disruption of glutamate receptors at the Shank postsynaptic platform could contribute to the destruction of the postsynaptic density, which underlies the synaptic dysfunction and loss in Alzheimer's disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cortactin SH3 domain-binding protein; Cortactin-binding protein 1; CortBP1; GKAP/SAPAP interacting protein; GKAP/SAPAP-interacting protein; multidomain postsynaptic density protein; PDZ-based scaffold protein; ProSAP1 protein; postsynaptic scaffold protein; proline rich synapse associated protein 1; proline rich synapse associated protein 2; proline-rich synapse associated protein 1; Proline-rich synapse-associated protein 1; Proline-rich synapse-associated protein 2; ProSAP1; ProSAP2; SAPAP-interacting protein synamon; SH3 and multiple ankyrin repeat domains 1; SH3 and multiple ankyrin repeat domains protein 1; SH3 and multiple ankyrin repeat domains protein 2; SH3 and multiple ankyrin repeat domains protein 3; SH3 and multiple ankyrin repeat domains protein 3 variant c-3; SH3 and multiple ankyrin repeat domains protein 3 variant c-4; SH3 and multiple ankyrin repeat domains-like protein 3 variant 1; SH3 and multiple ankyrin repeat domains-like protein 3 variant 2; SH3 and multiple ankyrin repeat domains-like protein 3 variant 3; SH3 and multiple ankyrin repeat domains-like protein 3 variant c-1; SH3 and multiple ankyrin repeat domains-like protein 3 variant c-2; SH3 and multiple ankyrin repeat domains-like protein 3 variant d-1; SH3 and multiple ankyrin repeat domains-like protein 3 variant d-2; SH3 and multiple ankyrin repeat domains-like protein 3 variant d-3; SH3 and multiple ankyrin repeat domains-like protein 3 variant e-1; SH3 and multiple ankyrin repeat domains-like protein 3 variant e-2; SH3/ankyrin domain gene 1; SH3/ankyrin domain gene 2; SH3/ankyrin domain gene 3; shank postsynaptic density protein; Shank postsynaptic density protein 3a; Shank1; Shank2; Shank3; shank3 postsynaptic density protein; shank3 splice variant II; shank3 splice variant III; shank3 splice variant IV; shank3e splice variant III; shank3e splice variant IV; Somatostatin receptor-interacting protein; SPANK-1; SPANK-2; SPANK-3; SSTR-interacting protein; Synamon
Gene Aliases: AI841104; AUTS17; CORTBP1; CTTNBP1; DEL22q13.3; KIAA1022; KIAA1650; mKIAA1022; PROSAP1; PROSAP2; PSAP2; SCZD15; SHANK; SHANK1; Shank1a; SHANK2; SHANK3; Shank3b; SHANK3c-3; SHANK3c-4; SPANK-1; SPANK-2; SPANK-3; Spank1; SSTRIP; synamon
UniProt ID: (Human) Q9Y566, (Human) Q9UPX8, (Human) Q9BYB0, (Rat) Q9WV48, (Mouse) D3YZU1, (Mouse) Q80Z38, (Rat) Q9QX74, (Mouse) Q4ACU6, (Rat) Q9JLU4
Entrez Gene ID: (Human) 50944, (Human) 22941, (Human) 85358, (Rat) 78957, (Mouse) 243961, (Mouse) 210274, (Rat) 171093, (Mouse) 58234, (Rat) 59312
G-protein coupled receptor binding
protein binding
SH3 domain binding
receptor signaling complex scaffold activity
GKAP/Homer scaffold activity
somatostatin receptor binding
ionotropic glutamate receptor binding
identical protein binding
macromolecular complex binding
ankyrin repeat binding
scaffold protein binding
structural constituent of postsynaptic density
actin binding
zinc ion binding
protein C-terminus binding
protein complex binding
protein self-association
MAPK cascade
embryonic epithelial tube formation
synapse assembly
learning
memory
striatal medium spiny neuron differentiation
adult behavior
negative regulation of actin filament bundle assembly
social behavior
locomotory exploration behavior
vocal learning
negative regulation of cell volume
positive regulation of long-term neuronal synaptic plasticity
brain morphogenesis
neuromuscular process controlling balance
protein oligomerization
positive regulation of synapse structural plasticity
positive regulation of synaptic transmission, glutamatergic
dendritic spine morphogenesis
positive regulation of dendritic spine development
regulation of dendritic spine morphogenesis
vocalization behavior
postsynaptic density assembly
AMPA glutamate receptor clustering
NMDA glutamate receptor clustering
guanylate kinase-associated protein clustering
regulation of long-term synaptic potentiation
positive regulation of long-term synaptic potentiation
positive regulation of glutamate receptor signaling pathway
regulation of long term synaptic depression
positive regulation of excitatory postsynaptic potential
regulation of grooming behavior
regulation of behavioral fear response
positive regulation of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity
associative learning
synapse organization
cognition
exploration behavior
long-term synaptic potentiation
long term synaptic depression
nervous system development
long-term memory
cell differentiation
protein localization to synapse
olfactory behavior
habituation
modulation of synaptic transmission
determination of affect
righting reflex
synapse maturation
protein-containing complex assembly
AMPA selective glutamate receptor signaling pathway
maintenance of postsynaptic density structure
positive regulation of cell proliferation
negative regulation of hippo signaling
protein complex assembly
cytoskeletal anchoring at plasma membrane
regulation of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity
brain development
response to nutrient
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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