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Invitrogen
Adenylate Kinase 4 Recombinant Rabbit Monoclonal Antibody (014)
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FIGURE: 1 / 1
Adenylate Kinase 4 Antibody (MA5-29020) in IP
Adenylate Kinase 4 Immunoprecipitation using: Lane A: 0.5 mg MCF-7 Whole Cell Lysate, Lane B: 0.5 mg HepG2 Whole Cell Lysate, Lane C: 0.5 mg 293T Whole Cell Lysate, Lane D: 0.5 mg A431 Whole Cell Lysate 2 µL with Adenylate Kinase 4 Recombinant Rabbit Monoclonal Antibody (14) (Product # MA5-29020) and 15 µL of 50 % Protein G agarose. Primary antibody: Adenylate Kinase 4 Recombinant Rabbit Monoclonal Antibody... View More
Product Details
MA5-29020
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
014
Immunogen
Recombinant Human AK4/AK3L1 protein (Ala2-Tyr223)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
This antibody has specificity for Human AK4/AK3L1.
Target Information
This gene encodes a member of the adenylate kinase family of enzymes. The encoded protein is localized to the mitochondrial matrix. Adenylate kinases regulate the adenine and guanine nucleotide compositions within a cell by catalyzing the reversible transfer of phosphate group among these nucleotides. Five isozymes of adenylate kinase have been identified in vertebrates. Expression of these isozymes is tissue-specific and developmentally regulated. A pseudogene for this gene has been located on chromosome 17. Three transcript variants encoding the same protein have been identified for this gene. Sequence alignment suggests that the gene defined by NM_013410, NM_203464, and NM_001005353 is located on chromosome 1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Adenylate kinase 3-like; adenylate kinase 3-like 1; Adenylate kinase 4, mitochondrial; adenylate kinase isoenzyme 4, mitochondrial; AK3; ATP-AMP transphosphorylase; epididymis secretory sperm binding protein; GTP:AMP phosphotransferase AK4; GTP:AMP phosphotransferase AK4, mitochondrial; mitochondrial adenylate kinase-3; nucleoside-triphosphate-adenylate kinase; RP4-686B20.1; unnamed protein product
Gene Aliases: AK 4; AK3; AK3L1; AK3L2; AK4
UniProt ID: (Human) P27144
Entrez Gene ID: (Human) 205
nucleotide binding
adenylate kinase activity
nucleoside diphosphate kinase activity
protein binding
ATP binding
GTP binding
kinase activity
transferase activity
phosphotransferase activity, phosphate group as acceptor
nucleobase-containing compound kinase activity
CMP kinase activity
dCMP kinase activity
nucleoside triphosphate adenylate kinase activity
dAMP kinase activity
nucleoside phosphate kinase activity
regulation of oxidative phosphorylation
nucleobase-containing compound metabolic process
ADP biosynthetic process
nucleotide metabolic process
nucleoside monophosphate metabolic process
nucleoside triphosphate biosynthetic process
ribonucleoside diphosphate biosynthetic process
nucleobase-containing small molecule interconversion
AMP metabolic process
ATP metabolic process
GTP metabolic process
nucleoside monophosphate phosphorylation
cellular response to hypoxia
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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