Search
Invitrogen
DNA Ligase IV Recombinant Rabbit Monoclonal Antibody (2T6J2)
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 1
DNA Ligase IV Antibody (MA5-35109) in WB
Western blot analysis of DNA Ligase IV in extracts of various cell lines. Samples were incubated with DNA Ligase IV Monoclonal antibody (Product # MA5-35109) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Product Details
MA5-35109
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
2T6J2
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 504-685 of human DNA Ligase IV (P49917)
Conjugate
Form
Liquid
Concentration
1.47 mg/mL
Amount
147 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: SRVGSGCTMK ELYDLGLKLA KYWKPFHRKA PPSSILCGTE KPEVYIEPCN SVIVQIKAAE IVPSDMYKTG CTLRFPRIEK IRDDKEWHEC MTLDDLEQLR GKASGKLASK HLYIGGDDEP QEKKRKAAPK MKKVIGIIEH LKAPNLTNVN KISNIFEDVE FCVMSGTDSQ PKPDLENRIA EF
Target Information
The protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: DNA joinase; DNA ligase 4; DNA ligase IV; DNA repair enzyme; ligase IV, DNA, ATP-dependent; Polydeoxyribonucleotide synthase [ATP] 4; polynucleotide ligase; sealase; unnamed protein product
Gene Aliases: LIG4; LIG4S
UniProt ID: (Human) P49917
Entrez Gene ID: (Human) 3981
single strand break repair
in utero embryonic development
pro-B cell differentiation
DNA repair
base-excision repair
nucleotide-excision repair, DNA gap filling
double-strand break repair
double-strand break repair via nonhomologous end joining
DNA recombination
cellular response to DNA damage stimulus
central nervous system development
cell proliferation
response to X-ray
response to ionizing radiation
response to gamma radiation
neurogenesis
T cell differentiation in thymus
V(D)J recombination
immunoglobulin V(D)J recombination
T cell receptor V(D)J recombination
somatic stem cell population maintenance
negative regulation of neuron apoptotic process
isotype switching
fibroblast proliferation
positive regulation of fibroblast proliferation
positive regulation of neurogenesis
chromosome organization
cell division
neuron apoptotic process
cellular response to lithium ion
cellular response to ionizing radiation
DNA biosynthetic process
stem cell proliferation
establishment of integrated proviral latency
double-strand break repair via classical nonhomologous end joining
DN2 thymocyte differentiation
positive regulation of chromosome organization
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support