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Invitrogen
KDEL Monoclonal Antibody (2C1), APC
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FIGURE: 1 / 2
KDEL Antibody (MA5-45869) in IHC (P)
Immunohistochemistry analysis using kidney. Fixation involved formalin fixed, paraffin embedded treatment. Samples were incubated with KDEL monoclonal antibody (Product # MA5-45869) at 1:25 for 1 hour at RT, followed by Goat Anti-Mouse IgG: Alexa Fluor 488. Counterstain used was Actin-binding Phalloidin-Alexa Fluor 633; DAPI (blue) nuclear stain. Magnification: 63X. (A) DAPI (blue) nuclear stain. (B) Phallo... View More
Product Details
MA5-45869
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
2C1
Immunogen
KDEL peptide (C-AVKDEL -COOH) conjugated at the N-terminal to Bovine Serum Albumin (BSA)
Conjugate
Excitation/Emission Max
651/660 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
95.46mM phosphate/2.48mM MES, pH 7.4, with 2mM EDTA
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
A 1:1,000 dilution of MA5-45869 was sufficient for detection of KDEL in 10 µg of HeLa cell lysates by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.|Detects KDEL tagged proteins.
Target Information
The sequence Lys-Asp-Glu-Leu (KDEL) or a closely related sequence, is present at the carboxy-terminus of soluble endoplasmic reticulum (ER) resident proteins and some membrane proteins. 78 and 94 kDa glucose regulated proteins, GRP78 and GRP94 respectively, and protein disulfide isomerase (PDI) all share the C-terminal KDEL sequence. The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 78 kDa glucose-regulated protein; 94 kDa glucose-regulated protein; 94 kDa glucose-regulated protein {ECO:0000250|UniProtKB:P08113}; Binding-immunoglobulin protein; BiP; chaperone; Endoplasmic reticulum chaperone BiP; endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; Endoplasmic reticulum resident protein 99; Endoplasmin; endoplasmin {ECO:0000250|UniProtKB:P08113}; endothelial cell (HBMEC) glycoprotein; epididymis luminal protein 35; epididymis secretory sperm binding protein Li 125m; epididymis secretory sperm binding protein Li 89n; ER lumen protein retaining receptor 1; ER lumen protein-retaining receptor 1; ERp99; glucose regulated protein, 78 kDa; glucose-regulated protein (grp78); glucose-regulated protein, 78kDa; gp96 homolog; GRP 78; GRP-78; GRP-94; heat shock 70 kDa protein 5; heat shock 70kD protein 5; heat shock 70kD protein 5 (glucose-regulated protein, 78kD); heat shock 70kDa protein 5 (glucose-regulated protein); heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa); Heat shock protein 70 family protein 5; Heat shock protein 90 kDa beta member 1; heat shock protein 90 kDa beta member 1 {ECO:0000250|UniProtKB:P08113}; heat shock protein 90kDa beta (Grp94), member 1; heat shock protein 90kDa beta family member 1; Heat shock protein family A member 5; Heat shock protein family C member 4; HSP70 family protein 5; hsp90b1 {ECO:0000250|UniProtKB:P08113}; Immunoglobulin heavy chain-binding protein; KDEL; KDEL (Lys-Asp-Glu-Leu) endoplasmic reticulum protein retention receptor 1; KDEL endoplasmic reticulum protein retention receptor 1; KDEL receptor 1; Lys-Asp-Glu-Leu; Polymorphic tumor rejection antigen 1; Putative MAPK-activating protein PM23; RP23-446N16.1; Steroidogenesis-activator polypeptide; stress-inducible tumor rejection antigen gp96; transforming growth factor alpha regulated gene 2; tumor rejection antigen (gp96) 1; Tumor rejection antigen 1; Tumor rejection antigen gp96; unnamed protein product; XAP-1 antigen
Gene Aliases: 8030486F04Rik; AL022860; AU019543; AW215843; baffled; BIP; D2Wsu141e; D2Wsu17e; ECGP; endoplasmin; ERD2; ERD2.1; ERp99; GP96; GRP78; GRP94; HDEL; HEL-S-125m; HEL-S-89n; HEL35; Hsce70; HSP90B1; HSPA5; HSPC4; KDELR1; mBiP; PM23; SEZ-7; Sez7; TA-3; Targ2; Tra-1; TRA1
UniProt ID: (Human) P14625, (Human) P11021, (Mouse) P08113, (Rat) Q66HD0, (Rat) P06761, (Mouse) P20029, (Human) P24390, (Mouse) Q99JH8, (Rat) Q569A6
Entrez Gene ID: (Human) 7184, (Human) 3309, (Mouse) 22027, (Rat) 362862, (Rat) 25617, (Mouse) 14828, (Human) 10945, (Mouse) 68137, (Rat) 361577
nucleotide binding
RNA binding
protein phosphatase inhibitor activity
calcium ion binding
protein binding
ATP binding
hydrolase activity
ATPase activity
protein phosphatase binding
protein binding involved in protein folding
low-density lipoprotein particle receptor binding
unfolded protein binding
ATP-dependent protein folding chaperone
enzyme binding
protein domain specific binding
protein serine/threonine kinase inhibitor activity
heat shock protein binding
ubiquitin protein ligase binding
ribosome binding
cadherin binding
chaperone binding
misfolded protein binding
virion binding
glycoprotein binding
KDEL sequence binding
ER retention sequence binding
molecular_function
Hsp90 family chaperone
membrane trafficking regulatory protein
response to hypoxia
protein folding
protein transport
positive regulation of Wnt signaling pathway
retrograde protein transport, ER to cytosol
actin rod assembly
positive regulation of toll-like receptor signaling pathway
protein folding in endoplasmic reticulum
response to endoplasmic reticulum stress
ERAD pathway
negative regulation of apoptotic process
sequestering of calcium ion
cellular response to manganese ion
cellular response to ATP
protein localization to plasma membrane
translational initiation
ER overload response
cerebellum structural organization
cerebellar Purkinje cell layer development
substantia nigra development
positive regulation of cell migration
negative regulation of transforming growth factor beta receptor signaling pathway
endoplasmic reticulum unfolded protein response
posttranslational protein targeting to membrane, translocation
negative regulation of protein complex assembly
positive regulation of protein ubiquitination
maintenance of protein localization in endoplasmic reticulum
IRE1-mediated unfolded protein response
PERK-mediated unfolded protein response
protein refolding
cellular response to glucose starvation
positive regulation of transcription from RNA polymerase II promoter
proteolysis involved in cellular protein catabolic process
regulation of protein folding in endoplasmic reticulum
cellular response to interleukin-4
regulation of ATF6-mediated unfolded protein response
regulation of IRE1-mediated unfolded protein response
negative regulation of IRE1-mediated unfolded protein response
regulation of PERK-mediated unfolded protein response
negative regulation of PERK-mediated unfolded protein response
response to stress
ER-associated ubiquitin-dependent protein catabolic process
regulation of phosphoprotein phosphatase activity
activation of signaling protein activity involved in unfolded protein response
cellular response to antibiotic
toxin transport
positive regulation of embryonic development
protein retention in ER lumen
retrograde vesicle-mediated transport, Golgi to ER
vesicle-mediated transport
T cell differentiation
T cell apoptotic process
transport
biological_process
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