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Invitrogen

KDEL Monoclonal Antibody (2C1), FITC

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KDEL Monoclonal Antibody (2C1), FITC

Product Details

MA5-45870

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:25
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

2C1

Immunogen

KDEL peptide (C-AVKDEL -COOH) conjugated at the N-terminal to Bovine Serum Albumin (BSA)
View immunogen

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra spectra

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

Protein G

Storage buffer

9.1mM sodium bicarbonate/PBS, pH 7.4, with 640.91mM DMSO, 136.36mM ethanolamine

Contains

no preservative

Storage conditions

4°C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2932324

Product Specific Information

A 1:1,000 dilution of MA5-45870 was sufficient for detection of KDEL in 10 µg of HeLa cell lysates by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.|Detects KDEL tagged proteins.

Target Information

The sequence Lys-Asp-Glu-Leu (KDEL) or a closely related sequence, is present at the carboxy-terminus of soluble endoplasmic reticulum (ER) resident proteins and some membrane proteins. 78 and 94 kDa glucose regulated proteins, GRP78 and GRP94 respectively, and protein disulfide isomerase (PDI) all share the C-terminal KDEL sequence. The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 78 kDa glucose-regulated protein; 94 kDa glucose-regulated protein; 94 kDa glucose-regulated protein {ECO:0000250|UniProtKB:P08113}; Binding-immunoglobulin protein; BiP; chaperone; Endoplasmic reticulum chaperone BiP; endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; Endoplasmic reticulum resident protein 99; Endoplasmin; endoplasmin {ECO:0000250|UniProtKB:P08113}; endothelial cell (HBMEC) glycoprotein; epididymis luminal protein 35; epididymis secretory sperm binding protein Li 125m; epididymis secretory sperm binding protein Li 89n; ER lumen protein retaining receptor 1; ER lumen protein-retaining receptor 1; ERp99; glucose regulated protein, 78 kDa; glucose-regulated protein (grp78); glucose-regulated protein, 78kDa; gp96 homolog; GRP 78; GRP-78; GRP-94; heat shock 70 kDa protein 5; heat shock 70kD protein 5; heat shock 70kD protein 5 (glucose-regulated protein, 78kD); heat shock 70kDa protein 5 (glucose-regulated protein); heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa); Heat shock protein 70 family protein 5; Heat shock protein 90 kDa beta member 1; heat shock protein 90 kDa beta member 1 {ECO:0000250|UniProtKB:P08113}; heat shock protein 90kDa beta (Grp94), member 1; heat shock protein 90kDa beta family member 1; Heat shock protein family A member 5; Heat shock protein family C member 4; HSP70 family protein 5; hsp90b1 {ECO:0000250|UniProtKB:P08113}; Immunoglobulin heavy chain-binding protein; KDEL; KDEL (Lys-Asp-Glu-Leu) endoplasmic reticulum protein retention receptor 1; KDEL endoplasmic reticulum protein retention receptor 1; KDEL receptor 1; Lys-Asp-Glu-Leu; Polymorphic tumor rejection antigen 1; Putative MAPK-activating protein PM23; RP23-446N16.1; Steroidogenesis-activator polypeptide; stress-inducible tumor rejection antigen gp96; transforming growth factor alpha regulated gene 2; tumor rejection antigen (gp96) 1; Tumor rejection antigen 1; Tumor rejection antigen gp96; unnamed protein product; XAP-1 antigen

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Gene Aliases: 8030486F04Rik; AL022860; AU019543; AW215843; baffled; BIP; D2Wsu141e; D2Wsu17e; ECGP; endoplasmin; ERD2; ERD2.1; ERp99; GP96; GRP78; GRP94; HDEL; HEL-S-125m; HEL-S-89n; HEL35; Hsce70; HSP90B1; HSPA5; HSPC4; KDELR1; mBiP; PM23; SEZ-7; Sez7; TA-3; Targ2; Tra-1; TRA1

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UniProt ID: (Human) P14625, (Human) P11021, (Mouse) P08113, (Rat) Q66HD0, (Rat) P06761, (Mouse) P20029, (Human) P24390, (Mouse) Q99JH8, (Rat) Q569A6

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Entrez Gene ID: (Human) 7184, (Human) 3309, (Mouse) 22027, (Rat) 362862, (Rat) 25617, (Mouse) 14828, (Human) 10945, (Mouse) 68137, (Rat) 361577

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Function(s)
nucleotide binding RNA binding protein phosphatase inhibitor activity calcium ion binding protein binding ATP binding hydrolase activity ATPase activity protein phosphatase binding protein binding involved in protein folding low-density lipoprotein particle receptor binding unfolded protein binding ATP-dependent protein folding chaperone enzyme binding protein domain specific binding protein serine/threonine kinase inhibitor activity heat shock protein binding ubiquitin protein ligase binding ribosome binding cadherin binding chaperone binding misfolded protein binding virion binding glycoprotein binding KDEL sequence binding ER retention sequence binding molecular_function Hsp90 family chaperone membrane trafficking regulatory protein
Process(es)
response to hypoxia protein folding protein transport positive regulation of Wnt signaling pathway retrograde protein transport, ER to cytosol actin rod assembly positive regulation of toll-like receptor signaling pathway protein folding in endoplasmic reticulum response to endoplasmic reticulum stress ERAD pathway negative regulation of apoptotic process sequestering of calcium ion cellular response to manganese ion cellular response to ATP protein localization to plasma membrane translational initiation ER overload response cerebellum structural organization cerebellar Purkinje cell layer development substantia nigra development positive regulation of cell migration negative regulation of transforming growth factor beta receptor signaling pathway endoplasmic reticulum unfolded protein response posttranslational protein targeting to membrane, translocation negative regulation of protein complex assembly positive regulation of protein ubiquitination maintenance of protein localization in endoplasmic reticulum IRE1-mediated unfolded protein response PERK-mediated unfolded protein response protein refolding cellular response to glucose starvation positive regulation of transcription from RNA polymerase II promoter proteolysis involved in cellular protein catabolic process regulation of protein folding in endoplasmic reticulum cellular response to interleukin-4 regulation of ATF6-mediated unfolded protein response regulation of IRE1-mediated unfolded protein response negative regulation of IRE1-mediated unfolded protein response regulation of PERK-mediated unfolded protein response negative regulation of PERK-mediated unfolded protein response response to stress ER-associated ubiquitin-dependent protein catabolic process regulation of phosphoprotein phosphatase activity activation of signaling protein activity involved in unfolded protein response cellular response to antibiotic toxin transport positive regulation of embryonic development protein retention in ER lumen retrograde vesicle-mediated transport, Golgi to ER vesicle-mediated transport T cell differentiation T cell apoptotic process transport biological_process
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