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Invitrogen
Phospho-MARK Pan (Thr215) Polyclonal Antibody
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FIGURE: 1 / 4
Phospho-MARK Pan (Thr215) Antibody (PA5-105814) in IHC (P)
Immunohistochemistry analysis of paraffin-embedded Phospho-MARK Pan (Thr215) in human kidney tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-MARK Pan (Thr215) polyclonal antibody (Product # PA5-105814) using a dilution of 1:200 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.
Product Details
PA5-105814
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human MARK1(Accession Q9P0L2), corresponding to amino acid residues around phosphorylated Thr215.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of MARK1/2/3/4 only when phosphorylated at Thr215.
Target Information
MARK1 (MAP/microtubule affinity-regulating kinase 1) belongs to the MARK family of serine/threonine kinases. MARK family protein kinases phosphorylate microtubule-associated proteins and may play a role in cytoskeletal stability. MARK2 refers to MAP/microtubule affinity-regulating kinase 2 isoform a [Homo sapiens]. EMK (ELKL Motif Kinase) is a small family of ser/thr protein kinases involved in the control of cell polarity, microtubule stability and cancer. Several cDNA clones have been isolated that encoded two isoforms of the human ser/thr protein kinase EMK1 called MARK2. MARK3 was originally identified as a marker that was induced by treatment with DNA damaging agents, and loss of MARK3 was found with carcinogenesis in the pancreas. MARK4 contains an N terminal serine/threonine kinase domain, a central ubiquitin associated domain, and a C terminal KA1 associated kinase domain. RT PCR analysis detects upregulated expression of the gene for MARK4 in nearly all clinical hepatocellular carcinoma cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C-TAK1; Cdc25C-associated protein kinase 1; cTAK1; ELKL motif kinase 1; ELKL motif kinase 2; ELKL motif serine-threonine protein kinase 3; ELKL motif serine/threonine-protein kinase 3; EMK-1; EMK-2; EMK1; EMK2/MARK3 serine-threonine protein kinase; epididymis secretory sperm binding protein; MAP/microtubule affinity-regulating kinase 1; MAP/microtubule affinity-regulating kinase 2; MAP/microtubule affinity-regulating kinase 3; MAP/microtubule affinity-regulating kinase 4; MAP/microtubule affinity-regulating kinase 4L; MAP/microtubule affinity-regulating kinase like 1; MAP/microtubule affinity-regulating kinase-like 1; mark1; mark1 {ECO:0000312|RGD:619882}; mark2 {ECO:0000312|RGD:708483}; MARK4 serine/threonine protein kinase; MPK-10; Par-1a; Par-1b; Par-1c; PAR1 homolog; PAR1 homolog b; PAR1 homolog c; protein kinase; Protein kinase STK10; Ser/Thr protein kinase PAR-1; Ser/Thr protein kinase PAR-1B; serine/threonine kinase; serine/threonine protein kinase EMK; Serine/threonine-protein kinase MARK1; Serine/threonine-protein kinase MARK2; Serine/threonine-protein kinase p78; testicular tissue protein Li 117; unnamed protein product
Gene Aliases: 1600015G02Rik; 2410090P21Rik; A430080F22Rik; AW491150; B930025N23Rik; C-TAK1; C79806; CTAK1; Emk; EMK-1; EMK1; EMK2; Emk3; ETK-1; ETK1; KIAA1477; KIAA1860; KP78; MARK; MARK1; MARK2; MARK3; MARK4; MARK4L; MARK4S; MARKL1; MARKL1L; mKIAA1477; mPar-1c; MPK10; PAR-1; Par-1a; Par-1b; Par-1c; PAR-1D; PAR1A; Par1b; Par1c; VIPB
UniProt ID: (Human) Q9P0L2, (Human) Q7KZI7, (Human) Q86TT8, (Human) Q96L34, (Mouse) Q8VHJ5, (Rat) O08678, (Rat) O08679, (Mouse) Q05512, (Rat) Q8VHF0, (Mouse) Q03141, (Mouse) Q8CIP4
Entrez Gene ID: (Human) 4139, (Human) 2011, (Human) 4140, (Human) 57787, (Mouse) 226778, (Rat) 117016, (Rat) 60328, (Mouse) 13728, (Rat) 170577, (Mouse) 17169, (Mouse) 232944, (Rat) 680407
nucleotide binding
magnesium ion binding
phosphatidylserine binding
protein kinase activity
protein serine/threonine kinase activity
protein binding
ATP binding
phosphatidylinositol-4,5-bisphosphate binding
lipid binding
kinase activity
transferase activity
metal ion binding
tau protein binding
tau-protein kinase activity
phosphatidic acid binding
protein serine kinase activity
RNA binding
protein kinase activator activity
cadherin binding
protein phosphatase binding
microtubule binding
cytoskeletal adaptor activity
gamma-tubulin binding
ubiquitin binding
transferase activity, transferring phosphorus-containing groups
poly(A) RNA binding
non-receptor serine/threonine protein kinase
microtubule cytoskeleton organization
neuron migration
protein phosphorylation
cytoskeleton organization
positive regulation of gene expression
negative regulation of gene expression
negative regulation of epithelial to mesenchymal transition
regulation of neuron projection development
Wnt signaling pathway
intracellular signal transduction
regulation of dendrite development
establishment of mitochondrion localization
regulation of postsynapse assembly
mitophagy
positive regulation of neuron projection development
establishment of cell polarity
cell differentiation
establishment or maintenance of epithelial cell apical/basal polarity
protein autophosphorylation
regulation of axonogenesis
regulation of cytoskeleton organization
mitochondrion localization
axon development
regulation of microtubule cytoskeleton organization
establishment or maintenance of cell polarity regulating cell shape
regulation of neurofibrillary tangle assembly
regulation of G2/M transition of mitotic cell cycle
negative regulation of hippo signaling
negative regulation of protein localization to nucleus
microtubule bundle formation
nervous system development
cell projection organization
positive regulation of programmed cell death
cilium organization
positive regulation of cilium assembly
positive regulation of cell cycle
regulation of centrosome cycle
microtubule polymerization
cell division
cilium disassembly
positive regulation of NLRP3 inflammasome complex assembly
positive regulation of protein localization to centrosome
phosphorylation
multicellular organism development
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