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Invitrogen
RhoGDI Polyclonal Antibody
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FIGURE: 1 / 6
RhoGDI Antibody (PA5-27413) in ICC/IF
Immunofluorescence analysis of RhoGDI was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 45 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with RhoGDI Polyclonal Antibody (Product # PA5-27413) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A3... View More
Product Details
PA5-27413
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human Rho GDI alpha. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.76 mg/mL
Amount
76 µg
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 10% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: NT2D, IMR32, Jurkat, HeLa, mouse brain.
Predicted reactivity: Mouse (97%), Rat (97%), Zebrafish (80%), Pig (97%), Chicken (90%), Chimpanzee (100%), Bovine (97%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Aplysia Ras-related homologs (ARHs), also called Rho genes, belong to the RAS gene superfamily encoding small guanine nucleotide exchange (GTP/GDP) factors. The ARH proteins may be kept in the inactive, GDP-bound state by interaction with GDP dissociation inhibitors, such as ARHGDIA.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ARHGDIA; epididymis secretory sperm binding protein Li 47e; GDI-1; GDP-dissociation inhibitor, aplysia RAS-related 1; MGC117248; Rho GDI 1; Rho GDI alpha; Rho GDP dissociation inhibitor (GDI) alpha; Rho GDP dissociation inhibitor (GDI) alpha isoform ARHGDIA_1; Rho GDP dissociation inhibitor (GDI) alpha isoform ARHGDIA_2; Rho GDP-dissociation inhibitor 1; Rho-GDI alpha; unnamed protein product
Gene Aliases: 5330430M07Rik; ARHGDIA; C87222; Gdi-1; Gdi1; GDIA1; HEL-S-47e; NPHS8; Rho-GDI; RhoDGI; RHOGDI; RHOGDI-1
UniProt ID: (Human) P52565, (Mouse) Q99PT1
Entrez Gene ID: (Human) 396, (Mouse) 192662
Rho protein signal transduction
regulation of protein localization
regulation of Rho protein signal transduction
negative regulation of apoptotic process
semaphorin-plexin signaling pathway
regulation of synaptic vesicle cycle
regulation of cell shape
negative regulation of cell migration
regulation of catalytic activity
cellular response to mechanical stimulus
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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