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Invitrogen

COX2 Polyclonal Antibody

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COX2 Polyclonal Antibody

Product Details

PA5-32366

Applications
Tested Dilution
Publications

Western Blot (WB)

1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:100
-

Immunocytochemistry (ICC/IF)

1:100
-

ELISA (ELISA)

0.5 µg/mL
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide derived from C-terminus of rat COX-2.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. not determined

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.6, with 1% BSA

Contains

0.1% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2549836

Product Specific Information

Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is colon, lung or breast carcinoma.

Target Information

COX2 converts arachidonate to prostaglandin H2 (PGH2), a committed step in prostanoid synthesis, including production of inflammatory prostaglandins. The conversion of arachidonate to prostaglandin H2 is a 2 step reaction: a cyclooxygenase (COX) reaction which converts arachidonate to prostaglandin G2 (PGG2) and a peroxidase reaction in which PGG2 is reduced to prostaglandin H2 (PGH2). It is constitutively expressed in some tissues in physiological conditions, such as the endothelium, kidney and brain, and is up-regulated under pathological conditions, such as in cancer and inflammation (in contrast to the iso-enzyme PTGS1, which is expressed ubiquitously). Up-regulation of COX2 is also associated with increased cell adhesion, phenotypic changes, resistance to apoptosis and tumor angiogenesis. In cancer cells, COX2 is a key step in the production of prostaglandin E2 (PGE2), which plays important roles in modulating motility, proliferation and resistance to apoptosis. COX2 is naturally inhibited by calcitriol (the active form of Vitamin D). Glucocorticoids chronically trans-repress PTGS2 gene activity in vivo in part by interfering with transcription initiation and elongation. COX2 is a target of NSAID such as aspirin, which can reduce pain and swelling from inflammation driven by COX2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: COX-2; cyclooxygenase; cyclooxygenase 2; cyclooxygenase 2 splice variant; cyclooxygenase 2b; Cyclooxygenase-2; Glucocorticoid-regulated inflammatory cyclooxygenase; Gripghs; Macrophage activation-associated marker protein P71/73; PES-2; PGH synthase 2; PHS II; Prostaglandin G/H synthase 2; Prostaglandin H2 synthase 2; prostaglandin-endoperoxide synthase; Prostaglandin-endoperoxide synthase 2; prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase); TIS10 protein; unnamed protein product

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Gene Aliases: COX-2; COX2; GRIPGHS; hCox-2; PGG/HS; PGHS-2; Pghs-b; Pghs2; PHS-2; PTGS2; TIS10

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UniProt ID: (Human) P35354, (Mouse) Q05769

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Entrez Gene ID: (Human) 5743, (Mouse) 19225

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Function(s)
peroxidase activity prostaglandin-endoperoxide synthase activity protein binding oxidoreductase activity oxidoreductase activity, acting on single donors with incorporation of molecular oxygen oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen enzyme binding heme binding protein homodimerization activity metal ion binding dioxygenase activity lipid binding oxygenase
Process(es)
prostaglandin biosynthetic process lipid metabolic process fatty acid metabolic process fatty acid biosynthetic process prostaglandin metabolic process response to oxidative stress embryo implantation regulation of blood pressure response to nematode response to selenium ion positive regulation of vascular endothelial growth factor production cyclooxygenase pathway lipoxygenase pathway positive regulation of prostaglandin biosynthetic process positive regulation of fever generation prostaglandin secretion regulation of cell proliferation long-chain fatty acid biosynthetic process negative regulation of apoptotic process positive regulation of nitric oxide biosynthetic process decidualization regulation of inflammatory response brown fat cell differentiation cellular response to hypoxia cellular response to non-ionic osmotic stress cellular response to fluid shear stress positive regulation of transforming growth factor beta production positive regulation of cell migration involved in sprouting angiogenesis positive regulation of fibroblast growth factor production positive regulation of brown fat cell differentiation positive regulation of platelet-derived growth factor production cellular oxidant detoxification regulation of neuroinflammatory response negative regulation of intrinsic apoptotic signaling pathway in response to osmotic stress angiogenesis inflammatory response learning memory positive regulation of cell proliferation negative regulation of cell proliferation positive regulation of cell death sensory perception of pain keratinocyte differentiation bone mineralization ovulation positive regulation of synaptic plasticity negative regulation of synaptic transmission, dopaminergic response to lipopolysaccharide response to cytokine maintenance of blood-brain barrier positive regulation of NF-kappaB import into nucleus anagen positive regulation of apoptotic process negative regulation of cell cycle positive regulation of vasoconstriction negative regulation of smooth muscle contraction positive regulation of smooth muscle contraction positive regulation of smooth muscle cell proliferation negative regulation of calcium ion transport positive regulation of synaptic transmission, glutamatergic oxidation-reduction process
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