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MFN1 Polyclonal Antibody
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FIGURE: 1 / 4
MFN1 Antibody (PA5-79665) in WB
Western blot was performed using MFN1 Polyclonal Antibody (Product # PA5-79665) and a 80 kDa band corresponding to MFN-1 was observed across cell lines and tissues tested. Whole cell extracts (30 µg lysate) of HeLa (Lane 1),A-431 (Lane 2), BeWo (Lane 3), PC-3 (Lane 4), Mouse heart (Lane5), Rat heart (Lane 6) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was ... View More
Product Details
PA5-79665
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Sheep
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminal of human Mitofusin 1 (23-49aa DQLLEFVTEGSHFVEATYKNPELDRIA).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: rat kidney tissue, rat heart tissue, mouse kidney tissue, mouse heart tissue, human placenta tissue.
Target Information
Mitofusin 1 (MFN1) and the related protein MFN2 are mitochondrial membrane GTPase proteins that play a central role in mitochondrial metabolism and may be associated with obesity and/or apoptosis processes. MFN1 and MFN2 form homotypic and heterotypic complexes and coordinately regulate mitochondrial fusion and are essential for embryonic development. When ectopically expressed, MFN1 inhibits the apoptosis-associated amino-terminal conformation change in the apoptotic protein Bax but not its mitochondrial translocation, indicating that MFN1 is involved in the regulating the activation of Bax on the outer mitochondrial membrane.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: DKFZp762F247; EC 3.6.5; FLJ20693; Fzo homolog; MFN1; putative mitochondrial transmembrane GTPase; MGC41806; mitochondrial transmembrane GTPase Fzo-1; mitochondrial transmembrane GTPase FZO-2; Mitochondrial transmembrane GTPase FZO1B; Mitofusin-1; putative transmembrane GTPase; similar to Drosophila fuzzy onions encoded by GenBank Accession Number U95821; Transmembrane GTPase MFN1; unnamed protein product
Gene Aliases: 2310002F04Rik; 6330416C07Rik; D3Ertd265e; Fzo1b; hfzo1; hfzo2; HR2; MFN1; mKIAA4032
UniProt ID: (Human) Q8IWA4, (Rat) Q8R4Z9, (Mouse) Q811U4
Entrez Gene ID: (Human) 55669, (Rat) 192647, (Mouse) 67414
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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