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Invitrogen
FLI1 Polyclonal Antibody
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FIGURE: 1 / 3
FLI1 Antibody (PA5-96374) in WB
Western Blot was performed using Anti-FLI1 Polyclonal Antibody (Product # PA5-96374) and a 50 kDa band corresponding to Friend leukemia integration 1 transcription factor was observed across all the cell lines tested except for the Raji cell line where a 55 kDa band was also observed. Nuclear enriched extracts (30 µg lysate) of MCF 10A (Lane 1), SK-BR-3 (Lane 2), MCF7 (Lane 3), Jurkat (Lane 4), HEL 92.1.7 (Lane 5), Raji (Lane 6), K-562 (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved pr... View More
Product Details
PA5-96374
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human FLI1 (NP_0020082)
Conjugate
Form
Liquid
Concentration
0.39 mg/mL
Amount
39 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: MDGTIKEALS VVSDDQSLFD SAYGAAAHLP KADMTASGSP DYGQPHKINP LPPQQEWINQ PVRVNVKREY DHMNGSRESP VDCSVSKCSK LVGGGESNPM; Positive Samples: THP-1, Jurkat, HepG2, Mouse heart; Cellular Location: Nucleus
Target Information
The FLI1 (Friend Leukemia Integration 1) gene is located on chromosome 11q24.3 and belongs to the ETS (E26 transformation-specific) family of transcription factors. Structurally, FLI1 contains an ETS domain responsible for DNA binding and a pointed domain which facilitates protein-protein interactions. Functionally, it plays a critical role in the regulation of embryonic and hematopoietic development. It is also implicated in the pathogenesis of diseases such as Ewing's sarcoma, wherein a chromosomal translocation t(11;22)(q24;q12) forms the EWS-FLI1 fusion gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: alternate; ETS transcription factor; Ewing sarcoma breakpoint region 2; Friend leukemia integration 1; Friend leukemia integration 1 transcription factor; Friend leukemia virus integration 1; Proto-oncogene Fli-1; Retroviral integration site protein Fli-1; similar to FLI-1; Transcription factor ERGB; unnamed protein product
Gene Aliases: BDPLT21; EWSR2; FLI-1; FLI1; SIC-1; Sic1
UniProt ID: (Human) Q01543, (Mouse) P26323
Entrez Gene ID: (Human) 2313, (Mouse) 14247
transcription regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
protein binding
sequence-specific DNA binding
sequence-specific double-stranded DNA binding
RNA polymerase II distal enhancer sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
winged helix/forkhead transcription factor
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
hemostasis
blood circulation
organ morphogenesis
cell differentiation
megakaryocyte development
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
transcription from RNA polymerase II promoter
cellular process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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