Proteintech

PTPN23 Polyclonal Antibody

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PTPN23 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( 4% PFA ) fixed HeLa cells using 10472-1-AP (PTPN23 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

PTPN23 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 10472-1-AP ( PTPN23 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

PTPN23 Antibody in Western Blot (WB) — HeLa cells were subjected to SDS PAGE followed by western blot with 10472-1-AP (PTPN23 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

PTPN23 Antibody in Immunoprecipitation (IP) — IP result of anti-PTPN23 (IP:10472-1-AP, 5ug; Detection:10472-1-AP 1:500) with HeLa cells lysate 1000ug.

Product Details

10472-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	View 8 publications 8 publications
Immunohistochemistry (IHC)	-	View 2 publications 2 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:400	-
Immunocytochemistry (ICC/IF)	1:50-1:500	View 3 publications 3 publications
Immunoprecipitation (IP)	0.5-4.0 µg	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PTPN23 Fusion Protein Ag0765 (420-619 aa encoded by BC004881) View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.38 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.1% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: QEKLHLRFC YEAVVRHVEQ VLQRHGVPPP CKPLASASIS QKNHLPQDSQ DLVLGGDVPI SSIQATIAKL SIRPPGGLES PVASLPGPAE PPGLPPASLP ESTPIPSSSP PPLSSPLPEA PQPKEEPPVP EAPSSGPPSS SLELLASLTP EAFSLDSSLR GKQRMSKHNF LQAHNGQGLR ATRPSDDPLS LLDPLWTLNK T (420-619 aa encoded by BC004881)

Target Information

Plays a role in sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs) via its interaction with the ESCRT-I complex (endosomal sorting complex required for transport I), and possibly also other ESCRT complexes. May act as a negative regulator of Ras-mediated mitogenic activity. Plays a role in ciliogenesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Concerted ESCRT and clathrin recruitment waves define the timing and morphology of intraluminal vesicle formation.

Nature communications

Wenzel EM,Schultz SW,Schink KO,Pedersen NM,Nähse V,Carlson A,Brech A,Stenmark H,Raiborg C

Thu Jul 26 00:00:00 EDT 2018

Proteomics reveals novel protein associations with early endosomes in an epidermal growth factor-dependent manner.

The Journal of biological chemistry

Gosney JA,Wilkey DW,Merchant ML,Ceresa BP

Fri Apr 20 00:00:00 EDT 2018

Proteomics reveals novel protein associations with early endosomes in an epidermal growth factor-dependent manner.

The Journal of biological chemistry

Gosney JA,Wilkey DW,Merchant ML,Ceresa BP

Fri Apr 20 00:00:00 EDT 2018

Suppression of protein tyrosine phosphatase N23 predisposes to breast tumorigenesis via activation of FYN kinase.

Genes & development

Zhang S,Fan G,Hao Y,Hammell M,Wilkinson JE,Tonks NK

Sun Oct 01 00:00:00 EDT 2017

Suppression of protein tyrosine phosphatase N23 predisposes to breast tumorigenesis via activation of FYN kinase.

Genes & development

Zhang S,Fan G,Hao Y,Hammell M,Wilkinson JE,Tonks NK

Sun Oct 01 00:00:00 EDT 2017

Autophagy-Independent Lysosomal Targeting Regulated by ULK1/2-FIP200 and ATG9.

Cell reports

Goodwin JM,Dowdle WE,DeJesus R,Wang Z,Bergman P,Kobylarz M,Lindeman A,Xavier RJ,McAllister G,Nyfeler B,Hoffman G,Murphy LO

Tue Sep 05 00:00:00 EDT 2017

Structural Basis for Specific Interaction of TGFβ Signaling Regulators SARA/Endofin with HD-PTP.

Structure (London, England : 1993)

Gahloth D,Levy C,Walker L,Wunderley L,Mould AP,Taylor S,Woodman P,Tabernero L

Wed Jul 05 00:00:00 EDT 2017

Tumor-suppressive function of protein-tyrosine phosphatase non-receptor type 23 in testicular germ cell tumors is lost upon overexpression of miR142-3p microRNA.

The Journal of biological chemistry

Tanaka K,Kondo K,Kitajima K,Muraoka M,Nozawa A,Hara T

Fri Aug 16 00:00:00 EDT 2013

Tumor-suppressive function of protein-tyrosine phosphatase non-receptor type 23 in testicular germ cell tumors is lost upon overexpression of miR142-3p microRNA.

The Journal of biological chemistry

Tanaka K,Kondo K,Kitajima K,Muraoka M,Nozawa A,Hara T

Fri Aug 16 00:00:00 EDT 2013

Recruitment of UBPY and ESCRT exchange drive HD-PTP-dependent sorting of EGFR to the MVB.

Current biology : CB

Ali N,Zhang L,Taylor S,Mironov A,Urbé S,Woodman P

Mon Mar 18 00:00:00 EDT 2013

The Bro1-domain-containing protein Myopic/HDPTP coordinates with Rab4 to regulate cell adhesion and migration.

Journal of cell science

Chen DY,Li MY,Wu SY,Lin YL,Tsai SP,Lai PL,Lin YT,Kuo JC,Meng TC,Chen GC

Mon Oct 15 00:00:00 EDT 2012

UBAP1 is a component of an endosome-specific ESCRT-I complex that is essential for MVB sorting.

Current biology : CB

Stefani F,Zhang L,Taylor S,Donovan J,Rollinson S,Doyotte A,Brownhill K,Bennion J,Pickering-Brown S,Woodman P

Tue Jul 26 00:00:00 EDT 2011

UBAP1 is a component of an endosome-specific ESCRT-I complex that is essential for MVB sorting.

Current biology : CB

Stefani F,Zhang L,Taylor S,Donovan J,Rollinson S,Doyotte A,Brownhill K,Bennion J,Pickering-Brown S,Woodman P

Tue Jul 26 00:00:00 EDT 2011

Bioinformatics

Protein Aliases: HD-PTP; His domain-containing protein tyrosine phosphatase; Protein tyrosine phosphatase TD14; PTP-TD14; Tyrosine-protein phosphatase non-receptor type 23

Gene Aliases: AI462446; HD-PTP; HDPTP; KIAA1471; PTP-TD14; PTPN23

UniProt ID: (Human) Q9H3S7, (Mouse) Q6PB44

Entrez Gene ID: (Human) 25930, (Mouse) 104831, (Rat) 117552

Molecular Function: membrane traffic protein protein phosphatase protein modifying enzyme