Proteintech

PTPN23 Polyclonal Antibody

Product Image Gallery

PTPN23 Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of ( 4% PFA ) fixed HeLa cells using 10472-1-AP (PTPN23 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

PTPN23 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 10472-1-AP ( PTPN23 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

PTPN23 Antibody in Western Blot (WB) — A375 cells were subjected to SDS PAGE followed by western blot with 10472-1-AP (PTPN23 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

PTPN23 Antibody in Immunoprecipitation (IP) — IP result of anti-PTPN23 (IP:10472-1-AP, 5ug; Detection:10472-1-AP 1:500) with HeLa cells lysate 1000ug.

Product Details

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC)

1:50-1:500

Immunofluorescence (IF)

1:50-1:500

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:400

Immunoprecipitation (IP)

0.5-4.0 µg

Western Blot (WB)

1:500-1:2000

View 8 publications 8 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

PTPN23 Fusion Protein Ag0765

Conjugate

Unconjugated

Form

Liquid

Concentration

0.38 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.1% sodium azide

Storage conditions

-20°C

Target Information

Plays a role in sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs) via its interaction with the ESCRT-I complex (endosomal sorting complex required for transport I), and possibly also other ESCRT complexes. May act as a negative regulator of Ras-mediated mitogenic activity. Plays a role in ciliogenesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: HD-PTP; His domain-containing protein tyrosine phosphatase; Protein tyrosine phosphatase TD14; PTP-TD14; Tyrosine-protein phosphatase non-receptor type 23

Gene Aliases: AI462446; HD-PTP; HDPTP; KIAA1471; PTP-TD14; PTPN23

UniProt ID: (Human) Q9H3S7, (Mouse) Q6PB44

Entrez Gene ID: (Human) 25930, (Mouse) 104831, (Rat) 117552

Molecular Function: hydrolase phosphatase protein phosphatase receptor transmembrane receptor regulatory/adaptor protein