Human metaphase chromosomes hybridized to centromere probes labeled with ChromaTide® OBEA-dCTP nucleotides.

Human metaphase chromosomes hybridized to centromere probes labeled with ChromaTide® OBEA-dCTP nucleotides. Probes specific to the a-satellite sequences from human chromosome 17, chromosome 15 and chromosome 1 were labeled using nick translation with ChromaTide® Alexa Fluor® 488-7-OBEA-dCTP (green; Cat. no. C21555), ChromaTide® Alexa Fluor® 594-7-OBEA-dCTP (red; Cat. no. C21558) and ChromaTide® Alexa Fluor® 647-12-OBEA-dCTP (pink; Cat. no. C21559), respectively. The probes were hybridized to metaphase spreads from peripheral blood lymphocyte cultures. The chromosomes were counterstained with Hoechst 33342 (blue) (Cat. no. H1399, H3570, H21492).

Human metaphase chromosomes hybridized to centromere probes labeled with ChromaTide® OBEA-dCTP nucleotides. Probes specific to the a-satellite sequences from human chromosome 17, chromosome 15 and chromosome 1 were labeled using nick translation with ChromaTide® Alexa Fluor® 488-7-OBEA-dCTP (green; Cat. no. C21555), ChromaTide® Alexa Fluor® 594-7-OBEA-dCTP (red; Cat. no. C21558) and ChromaTide® Alexa Fluor® 647-12-OBEA-dCTP (pink; Cat. no. C21559), respectively. The probes were hybridized to metaphase spreads from peripheral blood lymphocyte cultures. The chromosomes were counterstained with Hoechst 33342 (blue) (Cat. no. H1399, H3570, H21492).

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