Kits, Reagents, Accessories for Sanger Sequencing & Fragment Analysis
We provide optimized, streamlined kits to help you prepare samples for Sanger sequencing and fragment analysis applications on Applied Biosystems™ Genetic Analyzers, as well as a comprehensive selection of standards, capillaries, polymers, and other accessories for your genetic analyzer.
Use the tabs below to find the kits, reagents, and accessories to support your applications.
New–ExoSAP-IT Express reagent: PCR cleanup in 5 minutes
We continue to develop superior Sanger sequencing kits that help provide solutions for all your applications and templates. Click on the links below to order Applied Biosystems™ sequencing kits.
Learn more about our sequencing kits
Finding sequencing primers is simple and fast with our new Invitrogen™ Primer Designer™ tool for Sanger sequencing, containing >300,000 predesigned primer pairs for PCR and sequencing.
View predesigned primers for Sanger sequencing
To design your own custom primers for PCR and Sanger sequencing, use the Oligo Perfect Designer.
PCR and Sequencing Primers
The Applied Biosystems™ CleanSweep™ PCR Purification Reagent is used for enzymatic clean-up of an amplified PCR product in an easy-to-use, single pipetting step format. The reagent dephosphorylates unincorporated nucleotides and digests unused primers to ensure accurate downstream analysis of sequencing or single-nucleotide polymorphism (SNP) data.
Which sequencing reaction cleanup product is right for you?
| Cleanup Method | Description & Application | Product |
|---|---|---|
| Reagent-Based Sequencing Template Cleanup | Scavenges all unincorporated Applied Biosystems™ BigDye™ terminators. Stabilizes samples before analysis. Best for long and short fragment recovery. | |
| Gel Filtration Spin Columns | Recovers DNA fragments larger than 16 base pairs. Removes > 98% of salts, NTP's and other unwanted low-molecular-weight impurities. |
Fragment analysis techniques allow the development of multiple applications on the Genetic Analyzer. For an overview of the different applications visit the Fragment Analysis page.
We offer several kits for specific Applied Biosystems™ fragment analysis–based applications.
Fragment Analysis Kits
| Kits and Reagents | Applications | Associated Reagents |
|---|---|---|
| SNaPshot Multiplex System | Genotyping, BAC fingerprint, Methylation analysis | SNaPshot primer focus kits, GeneScan 120 LIZ Size Standard |
| AFLP Analysis System | Genome fingerprinting for microorganism or Genome fingerprinting for Plant |
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| Custom labeled primer | Microsatellite, LOH, chimerism, ISSR analysis, SSCP, RFLP, TRFLP, MSMSA |
Fragment Analysis Size Standards
| When DNA fragments are labeled with: | Choose a size standards labeled with: | Recommended Size Standard | Possible Applications | Other Kits and Products | |||
|---|---|---|---|---|---|---|---|
| dR110, dR6G, dTAMRA™, dROX (Dye Set DS-02 - Filter E5) | LIZ™ |
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| 5-FAM™, HEX™, NED™ (Dye Set DS-30 - Filter D) | ROX™ | GeneScan 500 ROX Size Standard | Custom Fragment Analysis | Custom Labeled Primer | |||
| 6-FAM™, VIC™, NED (Dye Set DS-31 - Filter D**) | ROX | GeneScan 500 ROX Size Standard | Custom Fragment Analysis |
Custom Labeled Primer | |||
| 5-FAM, JOE, NED (Dye Set DS-32 - Filter F) | ROX | GeneScan 500 ROX Size Standard |
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| 6-FAM, VIC, NED, PET™ (Dye Set DS-33 - Filter G5) | LIZ | GeneScan 600 LIZ Size Standard v2.0 |
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Resources
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Support
New Capillary Electrophoresis Instruments Support Center
Find tips, troubleshooting help, and resources for support related to your capillary electrophoresis instruments.
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