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Consider Semi-preparative Columns to Improve Your Oligonucleotide Analysis

By 07.30.2024

The field of oligonucleotide therapeutics is expanding due to the push toward personalized medicine and continued research into the development of therapies for rare diseases. However, oligonucleotides are difficult to produce in high yields with high purity and formulate for efficient delivery and stability. Are you faced with the challenge of effectively keeping up with the growing demand for oligonucleotide testing? Are you seeking a way to accurately separate and purify complex oligonucleotides samples? Here are two questions that will help you consider a new column format that is reliable and cost-effective:

How can I carry out oligonucleotide analysis in high yields and be cost-effective?

Your laboratory can experience easy integration into your existing setup and smooth scalability from analytical to semi-preparative scale at high throughput, without sacrificing consistent and reliable performance. The industry leading Thermo Scientific DNAPac RP HPLC Columns are now available in a semi-preparative format for oligonucleotide purification accessible on a semi-preparative LC system. The unique performance of the DNAPac RP microporous structure achieves efficient separation at a preparative scale, even with instruments operating up to 10 mL/min.

How would a semi-prep column format improve my oligonucleotide analysis?

The unique chemistry of the DNAPac RP semi-preparative column delivers unmatched performance across a wide range of pH, temperature, and mobile phase compositions, enabling precise separations and accurate quantification, regardless of your experimental conditions. Specifically designed for ion-pair reversed-phase (IP-RP) separations, the column’s polymeric backbone bead provides sharper peaks and less band broadening, which are often seen with silica columns. The column’s wide operating pH range (0-14) and high-temperature range (up to 110°C) make it the ideal support for method development.

The DNAPac RP column chemistry is based on a unique super-macroporous structure with a pore size range of up to 2000 Å, offering excellent separation of both short and long single- and double-stranded nucleic acids, including RNA modalities such as intact mRNA.

Its biocompatible inert stainless-steel hardware reduces undesired secondary interactions, increasing recovery and ensuring accurate and reproducible results. By reducing unwanted interactions, the column preserves the integrity of your samples and maintains the purity of your analytes.

To learn more about the DNAPac RP semi-preparative columns, check out these online resources:

Visit our LinkedIn page #oligonucleotides #DNAPac


Gilles Jaouen

Gilles Jaouen completed his Chemical Engineering Msc at Lille National Engineering School of Chemistry and MBA at Insead (France). Following graduation, he contributed to several market-leading groups on developing, marketing and managing chromatography columns and consumables portfolio. In 2022, he joined Thermo Fisher Scientific, where he currently leads the LC Columns Product Management team, including BioLC and DNA Pac column family.