Anyone in a lab running next generation sequencing (NGS) experiments knows what a challenge it is to keep track of the myriad samples, libraries, adapters, reagents, instruments and associated data. Trying to manually track these with spreadsheets and notebooks can prove to be a frustrating and erratic experience. We are scientists just like you, and we have experienced these same frustrations. As a result, we developed a reliable way to trace samples and experiments; an easy way to access the details of the reagents consumed, the instrumentation used, and the scientists who performed the experiments.
Producing High-Quality Libraries
NGS is an essential tool for researchers, and the quality of library preparation is a critical success factor. High-quality libraries generate high-quality results. With the many steps involved to produce high-quality libraries, it is imperative to track where in the workflow these samples are and minimize potential error and rework, all without a cumbersome amount of administrative overhead. The latest advances to the Library Prep capabilities in the NGS Workflow Solution for Thermo Scientific™ Core LIMS™ software streamlines data capture, linking sample metadata to assay data, and tracks sample progression through the workflow steps in real time. Specifically, researchers can now:
- Easily visualize library prep plates
- Add barcodes or adapters to samples using drag and drop capabilities
- Cherry pick samples from multiple containers for processing in parallel
Multiplexing and Pooling Libraries
Maximizing the cost-effectiveness of sequencing is another major concern for many labs. One of the best ways to maximize productivity around sequencing is to take full advantage of the capacity of a sequencing platform by multiplexing and pooling libraries. This can come with its own set of pitfalls. Normalizing the libraries so that each is equally represented in the pool is critical for successful data outcomes. And equally significant is ensuring that no index adapter or library barcode is duplicated within a pool, otherwise differentiating the data of multiplexed libraries would be impossible. The enhancements to the pooling capabilities with this the NGS Workflow solution now enable researchers to:
- Normalize libraries with automatically calculated dilution instructions
- Pool libraries using drag-and-drop functionality to maximize sequencing capacity
- Immediately spot index clashes
Pre-configured Solution for NGS
Core LIMS software provides a streamlined, pre-configured solution for NGS to track and manage data collection, from sample prep through sequencing, regardless of instrumentation. Samples progress through the NGS workflow process from one step to the next, automatically tracking chain of custody, parent/child relationships, and all metadata associated with each experiment. Integrated dashboards also provide insight into lab productivity in order to quickly spot any bottlenecks. NGS supports a broad range of applications and disciplines, and across these is a common need to collect, access and report data. The NGS Workflow Solution facilitates all aspects of data collection, access and reporting. It is designed to be intuitive and simple to use, thereby minimizing implementation timelines and maximizing productivity.
Read our brochure on our NGS Workflow Solutions to learn more about how we can support your genomic workflows.