TRIzol reagent is a widely used chemical solution for the extraction of RNA, DNA, and proteins from biological samples. It is a monophasic solution of phenol and guanidine isothiocyanate, which facilitates the breakdown of cells and the denaturation of proteins. TRIzol reagent works through a series of steps to isolate high-quality RNA from biological samples. Initially, when a sample is mixed with TRIzol, the cells are lysed, releasing cellular components into the solution. The phenol and guanidine isothiocyanate in TRIzol disrupt the cell membrane and denature proteins, protecting RNA from degradation by RNases. Following homogenization, chloroform is added, and the mixture is centrifuged, resulting in three distinct phases: the aqueous phase containing RNA, the interphase containing DNA, and the organic phase containing proteins and other cellular components. The RNA from the aqueous phase is then precipitated by mixing with isopropanol and collected via centrifugation. The resulting RNA pellet is washed with ethanol to remove impurities and dissolved in RNase-free water or buffer for further use. This effective process makes TRIzol reagent a versatile tool in molecular biology for isolating RNA, DNA, and proteins from a single sample.

Learn more about TRIzol related protocols:

TRIzol Plus RNA Purification Kit
TRI Reagent DNA/Protein Isolation Protocol


TRIzol selection guide

ProductTRIzol Reagent product photo
TRIzol Reagent		TRIzol LS Reagent product photo
TRIzol LS Reagent		TRIzol Plus RNA Purification System product photo
TRIzol Plus RNA Purification Kit		TRIzol Max Bacterial RNA Isolation Kit product photo
TRIzol Max Bacterial RNA Isolation Kit
DescriptionIsolates total RNA from tissues or cells, including lipid-rich and difficult samples. Though most commonly used to isolate RNA from it's aqueous phase, Trizol can also be used to isolate DNA and protein from it's organic phase.Designed for use with liquid samples, such as serum or virus preparations.Offers rapid isolation of total RNA by combining the lysis capability of TRIzol Reagent with the convenient RNA extraction technology of the Invitrogen PureLink RNA Mini Kit silica spin column.Offers rapid isolation of total RNA from gram-positive and gram-negative bacteria by combining the lysis capability of TRIzol Reagent with Max Bacterial Enhancement Reagent to inactivate endogenous RNases and promote protein denaturing.
Primary sample typeBacteria, blood, cells, plant samples, tissue, viral samples, yeastBlood, liquid samples (e.g., serum), viral samplesBacteria, cells, plant samples, tissue, yeastBacteria
Final productTotal RNA, transcriptome RNA, or micro RNA or DNA and proteinTotal RNA, transcriptome RNA, or micro RNA or DNA and proteinTotal RNA, transcriptome RNA, or micro RNATotal RNA, transcriptome RNA, or micro RNA
Product size100 mL or 200 mL100 mL or 200 mL100 mL or 200 mL100 preps or 200 preps
Purification time1 hour1 hour1 hour1 hour
ApplicationsCloning, northern blotting, nuclease protection assays, real-time quantitative PCR (qPCR), reverse transcriptase PCR (RT-PCR), cDNA library constructionCloning, northern blotting, nuclease protection assays, real-time quantitative PCR (qPCR), reverse transcriptase PCR (RT-PCR), cDNA library constructionMicroarray analysis, northern blotting, nuclease protection assays, real-time quantitative PCR (qPCR), reverse transcriptase PCR (RT-PCR), cDNA library constructionCloning, northern blotting, nuclease protection assays, real-time quantitative PCR (qPCR), reverse transcriptase PCR (RT-PCR), cDNA library construction

 

A great match for TRIzol reagents: the PureLink RNA Mini Kit

The PureLink RNA Mini Kit is a column-based kit used to isolate high-quality total RNA from a wide variety of sample types including animal and plant cells, tissue, bacteria, and yeast. The kit includes RNase-free lysis and wash solutions that help protect RNA from RNases while liberating the RNA from DNA, proteins, and other cellular debris.

Combine it with TRIzol Reagent for a easy, reliable, and rapid method for total RNA isolation, even from difficult samples such as fibrous or fatty tissues.

  • Process sample inputs from 5–200 mg of animal/plant tissue or from 0.5 x 106 to 108 tissue culture cells with one spin column in 20 minutes
  • Isolate more RNA than competitor mini spin columns: up to 1,000 μg of total RNA from one column
  • Use isolated RNA in most applications without the need for further DNase treatment (very low minimal residual DNA carry over)

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sho-trizol

TRIzol® 제품은 수천 건의 저널 논문에 참조 된 제품으로, 다양한 종류의 생물 물질에서 고품질 무손상 RNA를 제공하기 위해 분자 생물학자들이 TRIzol® 시약에 보인 신뢰가 고스란히 담겨 있습니다.

TRIzol® 시약은 즉시 사용 가능한 다양한 형식으로 출시되어 있습니다. TRIzol® 시약 제품군 내의 개별 제품은 total RNA를 분리하거나 인간, 동물, 식물, 효모, 박테리아, 바이러스 기원의 샘플 등 다양한 생물 공급원의 RNA, DNA, 단백질을 동시에 분리할 수 있도록 맞춤 제작되었습니다.

RNA 추출 제품 선택 가이드

제품설명일차 샘플 유형
Invitrogen™ TRIzol™ Plus RNA Purification SystemTRIzol® 시약의 용해력과 PureLink® RNA Mini Kit 실리카 스핀 컬럼의 편리한 RNA 추출 기능을 결합하여 total RNA를 신속하게 분리모든 샘플 유형 중에서도 특히 섬유질이나 지방 조직과 식물 물질을 비롯해 난해한 샘플
TRIzol Reagent지질이 풍부하고 난해한 샘플을 포함한 조직이나 세포에서 total RNA 분리조직 및 세포
Invitrogen™ TRIzol™ LS Reagent혈청이나 바이러스 제제와 같은 액체 샘플에서 사용혈청 및 바이러스 제제와 같은 액체 샘플
Invitrogen™ TRIzol® 및 Max Bacterial Enhancement Reagent Kit 기반 시스템과 함께 TRIzol® Max™ Bacterial RNA IsolationKit는 박테리아 세포에서 total RNA를 분리하는 데 사용박테리아 샘플


Featured videos using TRIzol reagents

Video: How to isolate RNA from tissue or cells

Learn how to isolate ultrapure total RNA within an hour, even from difficult samples with the TRIzol Plus RNA Purification Kit.

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