Invitrogen™

TRIzol™ LS Reagent

Catalog number: 10296010
Invitrogen™

TRIzol™ LS Reagent

Catalog number: 10296010
Catalog Number
10296010
also known as 10296-010
Unit Size
100 mL
Price (USD)
262.00
Your Price:
Availability
***
Quantity
Catalog Number
10296028
also known as 10296-028
Unit Size
200 mL
Price (USD)
452.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
10296010
also known as 10296-010
100 mL
262.00
Your Price:
***
10296028
also known as 10296-028
200 mL
452.00
Your Price:
***
Product Overview
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Additional Information
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TRIzol® LS Reagent is a complete, ready-to-use reagent optimized for the isolation of high-quality total RNA, or the simultaneous isolation of RNA, DNA, and protein from a variety of liquid samples. This monophasic solution of phenol and guanidine isothiocyanate is designed to isolate separate fractions of RNA, DNA, and proteins from liquid samples of human, animal, plant, yeast, bacterial, and viral origin, typically within one hour. Key features of TRIzol® LS Reagent:

• Formulated for use with liquid samples such as serum and virus preparations
• Facilitates recovery of RNA, DNA, and protein from a single liquid sample
• Offers excellent lysis capability, even with difficult biological fluids

Reliably purify RNA from multiple sample sources
TRIzol® LS Reagent is designed for processing a variety of liquid samples of up to 0.25 mL in volume. TRIzol® LS Reagent differs from the standard TRIzol® Reagent in concentration, which permits larger samples to be processed. Just as with the standard TRIzol® Reagent, the integrity of resulting RNA preparations is maintained by the highly effective inhibition of RNase activity during sample homogenization. The simplicity of the TRIzol® LS Reagent method allows simultaneous processing of a large number of samples. The entire procedure can be completed in 1 hour. Total RNA isolated by TRIzol® LS Reagent is free of protein and DNA contamination.

Formulated to serve multiple isolations
TRIzol® LS Reagent allows you to perform sequential precipitation of RNA, DNA, and proteins from a single sample. After homogenizing the sample with TRIzol® LS Reagent, chloroform is added, and the homogenate is allowed to separate into a clear upper aqueous layer (containing RNA), an interface, and a red lower organic layer (containing the DNA and proteins). RNA is precipitated from the aqueous layer with isopropanol. DNA is precipitated from the aqueous/organic interface with ethanol. Protein is precipitated from the phenol-ethanol layer by isopropanol precipitation. The precipitated RNA, DNA, or protein is washed to remove impurities, and then resuspended for use in downstream applications.

Purified products are ideal for use with a variety of applications
Isolated RNA can be used in real-time quantitative PCR (qPCR), northern blot analysis, dot blot hybridization, poly-(A)+ selection, In vitro translation, RNase protection assays, and molecular cloning. Isolated DNA can be used in PCR, restriction enzyme digestion, and Southern blots. Isolated protein can be used for western blots, recovery of some enzymatic activity, and some immunoprecipitation
For Research Use Only. Not for use in diagnostic procedures.

Specifications

No. of Samples
100
Quantity
1
Yield
8 mg
High-throughput Compatibility
Not High-throughput Compatible (Manual)
Shipping Condition
Room Temperature
Starting Material Amount
250 µl to 1 ml liquid samples (suggested range; scalable)
System Type
TRIzol
Product Line
TRIzol™
For Use With (Application)
Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), cDNA Library Construction, Nuclease Protection Assays, Northern Blotting, Cloning
Final Product Type
Total RNA, Transcriptome RNA, micro RNA
Purification Target
Total RNA, Transcriptome RNA, miRNA
Sample Type
Liquid Samples (e.g. Serum), Viral Samples, Blood
Purification Time
1 hr
No. of Reactions
100 Preps
Isolation Technology
Organic Extraction
Scale
Scaleable

Contents & Storage

One bottle TRIzol™ LS Reagent. Store at 2°C to 25°C.

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Frequently asked questions (FAQs)

Citations & References