Nucleic acid staining of Staphylococcus aureus.

Staphylococcus aureus is surface-labeled with green-fluorescent Alexa Fluor® 488 wheat germ agglutinin (Cat. No. W11261) and stained with SYTO® 59 red-fluorescent nucleic acid stain (Cat. No. S11341). A series of z-section images was acquired with a Deltavision wide-field optical sectioning microscope (Applied Precision, Inc.). A three-dimensional projection movie was generated from a deconvolved z-image stack.

Staphylococcus aureus is surface-labeled with green-fluorescent Alexa Fluor® 488 wheat germ agglutinin (Cat. No. W11261) and stained with SYTO® 59 red-fluorescent nucleic acid stain (Cat. No. S11341). A series of z-section images was acquired with a Deltavision wide-field optical sectioning microscope (Applied Precision, Inc.). A three-dimensional projection movie was generated from a deconvolved z-image stack.

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