Zero Blunt® Cloning Kits
The Zero Blunt® PCR Cloning Kit is designed to clone blunt PCR fragments (or any blunt DNA fragment) with a low background of non-recombinants. The pCR®-Blunt vector contains the lethal E. coli ccdB gene fused to the C-terminus of LacZα.
Ligation of a blunt PCR fragment disrupts expression of the lacZα-ccdB gene fusion permitting growth of only positive recombinants upon transformation. Cells that contain non-recombinant vector are killed when the transformation mixture is plated.
Speed with Flexibility
With the addition of the ExpressLink™ T4 DNA Ligase into Zero Blunt® Cloning Kits, ligation can now be performed at room temperature in only 5 minutes with reactions typically yielding >80% recombinants containing inserts.
The pCR™-Blunt Vector Features:
Performance and Value
|Zero Blunt® Cloning Kits|
|Cloning efficiency||95% (with control)|
|Time for ligation||5 minutes|
|Temperature for ligation||Room Temperature|
|PCR Clean-up required?||No|
|Selection Antibiotic||Kanamycin & Zeocin™|
|Forward and reverse sequencing primers|
|Control DNA template|
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.