Gibco Image Contest 2025 Winners

The 2025 Gibco Calendar Image Contest has officially wrapped up, and our winners have been selected. We received an impressive array of submissions spanning cancer research, 3-D printed tissue, neuroscience and more. Each image highlighted the intrinsic beauty of its scientific field while offering a distinctive glimpse into the researchers’ work. Thank you to all who participated.

Sepideh Cheheltani, University of Delaware, USA

Sepideh Cheheltani headshot image

About me

I am a PhD/MBA candidate in Biological Sciences at the University of Delaware, where my research focuses on the actin cytoskeleton and biomechanics of the ocular lens. My work examines how actin-associated proteins shape lens fiber-cell architecture and overall lens stiffness and clarity.

 

Throughout my PhD, I have become passionate about microscopy, especially confocal imaging, as a way to visualize cellular organization and protein localization.

 

My long-term goal is to combine scientific expertise and communication with strategic thinking to advance impactful work at the intersection of research, innovation, and human health.

Research behind the featured image

 

The winning image captures the anterior suture of an 8-week-old mouse ocular lens, where elongated fiber cells meet in a precise Y-shaped pattern. Proper alignment at these sutures is essential for lens transparency and mechanical function.

To create the image, I used whole-mount staining of the lens with rhodamine–phalloidin to visualize F-actin, WGA to label cell membranes and the capsule, and Hoechst stain to identify nuclei. This work is part of my current research on how the actin cytoskeleton organizes fiber-cell architecture and how disruptions in actin-binding proteins, such as CAP2, affect lens stiffness and biomechanics. 

Microscopic view of ocular lens anterior suture

Research behind the featured image

The winning image captures the anterior suture of an 8-week-old mouse ocular lens, where elongated fiber cells meet in a precise Y-shaped pattern. Proper alignment at these sutures is essential for lens transparency and mechanical function.

To create the image, I used whole-mount staining of the lens with rhodamine–phalloidin to visualize F-actin, WGA to label cell membranes and the capsule, and Hoechst stain to identify nuclei. This work is part of my current research on how the actin cytoskeleton organizes fiber-cell architecture and how disruptions in actin-binding proteins, such as CAP2, affect lens stiffness and biomechanics. 

Benedikt Geier, Stanford University, USA

Benedikt Geier headshot image

About me

I am fascinated by interactions between bacteria and host cells in animals and humans, spanning from symbiotic relationships in the deep sea—what I studied during my PhD—to infectious diseases such as Helicobacter pylori in the human stomach, which I am currently studying in my postdoc. The core of my research is how spatial organization and site-specific chemistry shape these host–microbe interactions, and I am developing tools that enable us to view these interactions from new perspectives. For example, by combining mass spectrometry imaging (MSI) with fluorescence microscopy, I can determine which metabolites are produced by bacteria and host cells within infected tissues at a spatial resolution of tens of micrometers.

Research behind the featured image

At Stanford, I study Helicobacter pylori, a bacterium that infects the human stomach and is linked to ulcers and gastric cancer. To model colonization of the stomach epithelium, I use an apical-out organoid model (as shown in the image) that enables infection of patient-derived organoids. High-resolution imaging of these infected organoids—small spheres of stomach epithelium—reveals how H. pylori attaches and forms microcolonies on the epithelial surface, enabling exceptional detail of these micrometer-scale interaction.

Peter Berenstein, PhD, Massachusetts General Hospital, USA

Peter Berenstein headshot image

About me

Bioengineer, Stem Cell Researcher and BioTech Entrepreneur.

 

My research career started at Bar-Ilan University with a fascinating study of neuromuscular diseases and use of Stem Cells and Extracellular Vesicles for their therapy. My next steps (during COVID outbreak) lead me to the industry, where I was employed as R&D Lead and Senior Scientist in several Biotech start-up companies. Eventually, I was fortunate to join as a Research Fellow at the Department of Dermatology at Mass General Hospital in Boston, MA, where I am happy to develop novel Bioengineering and Biophysical techniques for tissue regeneration and laser-based therapeutics. Microscopy and Imaging are the fields I love the most, since that’s the best way to make the science accessible and easier to understand for everyone.

Research behind the featured image

I was working on building the 3D-printed skeletal muscle tissue, trying to induce myogenic differentiation of bio-printed muscle stem cells. The process demanded weeks of calibration for every step of the experiment, and hours of microscopy to document and analyze every experimental condition, one of which you can see here.

Joshua Daoud, Queen Mary University of London, UK

Joshua Daoud headshot image

About me

I am a first-year PhD student studying Medical Engineering at Queen Mary University of London. My current research is focused on the development of a model of glioblastoma (a life-limiting brain tumour diagnosis) using microfluidic chips to investigate how tumour growth mechanisms are influenced by the surrounding microenvironment in the brain. 


Using these microfluidic models, we aim to better our understanding of what drives the recurrence of glioblastoma tumours and how this can be targeted through future treatments and interventions. 

Research behind the featured image

In my image, I have observed how astrocytes (the most abundant cells in the brain) interact when grown alongside brain tumour cells. This research has informed further experiments to investigate what drives their interactions and how this may explain why glioblastoma tumours often recur.

Swetha Gurumurthy, ALS Therapy Development Institute, USA

Swetha Gurumurthy headshot image

About me

I am currently an Associate Scientist II at the ALS Therapy Development Institute, where I collaborate with Dr. Kyle Denton to investigate the underlying pathological mechanisms of amyotrophic lateral sclerosis (ALS) using immunocytochemistry and high-content neuronal imaging. My academic background includes a bachelor’s degree in biotechnology and a master’s in human physiology, both of which sparked my interest in neuroscience and electrophysiology. Since then, I have developed expertise in immunocytochemistry, neuronal differentiation, and high-content imaging. In my current role, I focus on applying these approaches to study disease-relevant mechanisms. Looking ahead, I aim to continue contributing to research that advances our understanding of ALS and supports the development of potential therapeutic strategies.

Research behind the featured image

To study the underlying pathophysiology of amyotrophic lateral sclerosis (ALS), human induced pluripotent stem cells (iPSCs) were differentiated into motor neurons and stained for the neuronal marker Tuj-1 (red, β-III tubulin) and nuclei using DAPI (blue). These iPSC-derived motor neuron cultures serve as a powerful platform for modeling neurodegenerative disease phenotypes and screening potential therapeutic compounds in a human cellular context.

The featured image shows immunostaining of iPSC-derived motor neurons with Tuj-1 (β-III tubulin, red) to visualize neuronal processes and DAPI (blue) to label nuclei, captured at 20x magnification using an Agilent BioTek Cytation C10 confocal imaging reader. Following paraformaldehyde (PFA) fixation, permeabilization, blocking, and primary and secondary antibody incubation, a z-stack of 10 slices was acquired and merged using a z-projection to generate the final composite image, highlighting the intricate architecture of the neurite network.

Swetha with favorite Gibco products

A selfie with my favorite Gibco products: Gibco Neurobasal and DMEM/F-12 media, supplemented with N2 and B-27, which play a central role in our iPSC-based neuronal differentiation protocol by providing the essential nutrients and optimized environment needed to reproducibly generate healthy neuronal cultures for ALS disease modeling.

Syed Sajidul Islam, Cooper Medical School, Rowan University, USA

Syed Sajidul Islam headshot image

About me

I am currently pursuing a PhD in Neuroscience at Cooper Medical School of Rowan University, working in Dr. Ben Sorum’s lab. My primary research focuses on mechanosensitive potassium channels; specifically, how mechanical stimulation rapidly activates these channels to modulate potassium flux and influence neuronal activity on a microsecond timescale. I am also involved in additional projects, including traumatic brain injury research using non-invasive focused ultrasound and neuromodulation approaches.

Research behind the featured image

The primary objective of this project was to develop a novel traumatic brain injury model using focused ultrasound. This image shows brain sections from C57 mice stained for microglia and astrocytes. The staining allowed us to examine cell morphology and structural features to assess whether microglia, the brain’s resident immune cells, exhibit an activated phenotype. This analysis was performed to determine whether microglia exhibited resting or activated phenotypes in response to experimental conditions.

Experimental details: mice were anesthetized and transcardially perfused with phosphate-buffered saline (PBS), followed by 4% paraformaldehyde (PFA) for tissue fixation. Brains were extracted, post-fixed overnight, cryoprotected in 30% sucrose, and frozen prior to sectioning. Coronal brain sections (20 µm) were cut using a cryostat. Sections were washed in PBS, then permeabilized and blocked using goat serum containing Triton X-100 to minimize non-specific binding. To assess neuroinflammatory responses, microglia were labeled with an Iba1 primary antibody, and astrocytes were labeled with a GFAP primary antibody. Sections were incubated with primary antibodies overnight at 4°C to ensure adequate penetration. After PBS washes the following day, fluorophore-conjugated secondary antibodies were applied for 2 h at room temperature, followed by DAPI counterstaining to visualize nuclei. Tissues were mounted with an anti-fade medium and imaged using a Leica upright fluorescence microscope. High-resolution image tiles (>30 per sample) were acquired and subsequently stitched using image-analysis software to generate composite images of the entire coronal brain.

Onon Batnyam, London Health Science Centre, Canada

Onon Batnyam headshot image

About me

I earned my PhD in Japan, my master’s in Taiwan, and my bachelor’s in Mongolia. Having studied across several countries, including time in Russia and Canada, I’m driven by curiosity, a love of travel, and a passion for challenging myself in new environments and fields.

 

The Pulmonary Injury, Inflammation, and Repair Lab (PIIRL) aims to understand how inflammation resolves and tissue repair occurs following lung injury, especially in the context of Acute Respiratory Distress Syndrome (ARDS), by identifying key mediators of microvascular endothelial cell (MVEC) dysfunction and inflammation, with the ultimate goal of discovering novel therapeutic targets. Our projects include studying how enzymes like metalloproteinases and their inhibitors (TIMPs) regulate MVEC dysfunction, macrophage behaviour, and fibrosis, as well as how caspase-mediated cell death contributes to endothelial injury in models of sepsis, infection or chemical insult, under different conditions such as aging or exercise. To contribute to such goals in our own lab, we isolate mouse endothelial cells and confirm their identity by immunostaining with the tight-junction marker Claudin 5, thereby ensuring we work with bona fide endothelial cells for downstream experiments.

Research behind the featured image

We isolate mouse endothelial cells and verify their identity by Claudin-5 immunostaining to ensure we are using bona fide endothelial cells in downstream experiments.

Joanne Lee, Vanderbilt University, USA

Joanne Lee headshot image

About me

I recently graduated with my PhD in biomedical engineering from Vanderbilt University in May 2025. While at Vanderbilt, I worked in Dr. Jonathan Brunger’s lab where I engineered cells with synthetic receptors to respond to native inflammatory cytokines to address regenerative medicine and Parkinson’s disease. Currently, I am working as a project manager in regulatory affairs.

Research behind the featured image

Biomaterials are commonly used in the field of regenerative medicine to present cues to cells to promote regeneration and reduce inflammation. In this image, I have engineered L929 mouse fibroblast cells to express a synthetic receptor sensitive to GFP. These cells express BFP constitutively for ease of visualization. I then exposed the cells to a bead decorated with a nanobody on the surface that is sensitive to RFP and supplemented the media with a GFP:RFP fusion protein. In this way, the bead should bind to the RFP half of the fusion protein and display the GFP half to the synthetic cell receptor for binding and activation. When the synthetic receptor is activated, the cells will produce fluorescent mCherry protein. We utilized this system as a proof of concept to demonstrate how we could co-engineer biomaterial surfaces decorated with nanobodies to assist in activating synthetic receptors. By creating systems where biomaterials and cells can work together effectively, we hope to more precisely control cell behaviors for regenerative medicine.

Pei-Chen Wu, Duke University, USA

Pei-Chen Wu headshot image

About me

I am currently a fifth-year PhD candidate with a long-standing interest in how metabolism changes in disease. My research focuses on understanding metabolic regulation in cardiovascular biology, with a particular emphasis on the role of small nucleolar RNAs (snoRNAs). I am especially interested in how these non-coding RNAs influence cardiac physiology and contribute to molecular changes during stress or disease. Throughout my academic journey, I’ve been driven by a curiosity about how metabolic pathways adapt and how these adaptations can guide new therapeutic strategies.

Research behind the featured image

The image I submitted comes from a project exploring how specific snoRNAs affect heart physiology. As part of this work, we examine cardiac hypertrophy in mouse models. The featured image is a wheat germ agglutinin (WGA) staining of a cross-section of the left ventricle from a 10-week-old mouse. This staining allows us to visualize cardiomyocyte boundaries and assess changes in cell size, which is critical for evaluating hypertrophic remodeling. 

Youlim Kim, Stanford University, USA

Youlim Kim headshot image

About me

I am currently a PhD candidate in Microbiology and Immunology at Stanford University. I have been working in the Amieva lab since 2020 and have learned to see a lot by just looking. I had never done fluorescent microscopy before joining the Amieva lab, but am excited to now take my training into a postdoc in academia or in industry to continue seeing the unseen.

Picture with my labmate.

A picture with my labmate, Benedikt Geier, who also had his image selected for the calendar!

Research behind the featured image

My research focuses on characterizing the responses of epithelial cells to bacterial infection, namely Helicobacter pylori's effect on the gastric epithelium. I study this using human in vitro model systems such as organoids, and I rely on imaging to tell me not only how the bacteria alters its host, but also how the host impacts bacterial colonization. This image displays the ability of H. pylori to form clonal microcolonies on apical-out 3D gastric organoids, growing out from an initial founder bacterium within 24 hours.

Helene Lubrano, Institute of Molecular and Cellular Pharmacology, France

Helene Lubrano headshot image

About me

I started my PhD in 2023 after completing a Master's degree in Neurosciences and Signalling in France with various placements. One of my placements sparked a vivid interest in ion channels and electrophysiology which is what I decided to continue doing for my thesis work. I am currently starting my 3rd (and probably last) year of PhD which is about the roles of specific ion channels—called ASICs (Acid-Sensing Ion Channels)—and lipids in pain. During that project I did mainly electrophysiology but eventually started dedicating quite a lot of time to microscopy to study the impact of lipids on membranes.

Research behind the featured image

This shot was taken on mouse DRG neurons while monitoring membrane tension with a specific fluorescent probe during lipids applications.

With this shot and still a long way ahead before I finish my thesis, I hope to be able to give a bit more insight on the interaction between lipids, ion channels and pain!

For Research Use Only. Not for use in diagnostic procedures.