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Invitrogen
Human IL-8 ELISA Development Kit (TMB), PeproTech®
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Product Specifications
Analytical sensitivity
Lot-specific
Assay range
Lot-specific
Hands-on time
1 hr 45 min
Time-to-result
24 hr 30 min
Homogenous (no wash)
No
Instrument
Absorbance Microplate Reader
Product size
2 x 96 Tests
Contents
This Human IL-8 (CXCL8) ELISA Kit includes: Capture Antibody, Detection Antibody, Protein Standard, and HRP-Conjugate.
Shipping conditions
Ambient
Storage
Refer to product documentation for component specific storage temperature.
Protein name
IL-8
Target site-specificity
Protein family
Growth Factors & Receptors
Assay kit format
ELISA Development Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
Human IL-8 ELISA development kit contains the key components required for the quantitative measurement of natural and/or recombinant human IL-8 in a sandwich ELISA format. Using the ELISA protocol, the recommended microplates, reagents and solutions, the components supplied in this kit are sufficient to assay human IL-8 in approximately 200 ELISA plate wells.
This PeproTech TMB ELISA Development Kit contains the key components required for the quantitative measurement of natural and/or recombinant proteins in a sandwich ELISA format. This TMB ELISA Development Kit contains enough material to assay the target cytokine in at least 200 ELISA plate wells. The TMB ELISA Buffer Kit (Product # 900-T00), sold separately, is recommended to be used in conjunction with this TMB ELISA Development Kit for optimal performance. This buffer kit contains all the necessary components to assay ten 96-well ELISA plates (included) and contains detailed handling instructions.
Target information
Interleukin 8 (IL-8, CXCL8) is a 72 amino acid pro-inflammatory factor which belongs to the CXC subfamily of chemokines, and are bound by the cell surface receptors IL-8RA and IL-8RB. IL-8 functions as a chemoattractant and potent angiogenic factor. The expression and secretion of IL-8 can be induced by diverse inflammatory stimuli in many cells, including macrophages and endothelial cells. In endothelial cells, IL-8 is present in storage vesicles called Weibel-Palade bodies. IL-8, first isolated from osteosarcoma cells, contains the ELR-motif (N-terminal Glu-Leu-Arg amino acid sequence) and signals through the CXCR1 and CXCR2 receptors. Previous nomenclature for IL-8 includes neutrophil activating protein 1 (NAP-1), granulocyte chemotactic protein 1 (GCP-1), monocyte-derived neutrophil-activating peptide (MONAP) and protein 3-10C. IL-8 and ten other members of the CXC chemokine gene family form a chemokine gene cluster in a region mapped to chromosome 4q. Cancer studies have demonstrated a role for IL-8 in the angiogenesis and growth of tumours, and IL-8 is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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