The Rat Prolactin Recepto) ELISA quantitates Rt Prolactin Receptor in rat serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Rt Prolactin Receptor.
Principle of the method
The Rat Prolactin Receptor solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, binding to the target on a different epitope from the capture antibody. A conjugated enzyme has been incorporated into the assay. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Prolactin (PRL) is a neuroendocrine hormone that is closely related to growth hormones and more distantly related to the peptide hormones of the interleukin family. The endocrine effects of PRL on human breast tissues include the regulation of growth and differentiation of ductal epithelium, proliferation and differentiation of lobular units, and initiation and maintenance of lactation. It is also involved in a variety of important functions including ion transport and osmoregulation, stimulation of milk, protein synthesis as well as the regulation of numerous reproductive functions. PRL exerts its influence on different cell types through a signal transduction pathway which begins with the binding of the hormone to a transmembrane PRL receptor. Immunoreactive PRL receptor, a member of the cytokine receptor family, varies in size (short and long forms) with tissue source and species, from ~40 kDa to 100 kDa. PRL and growth hormone receptors are members of the cytokine receptor superfamily. Both the PRL and growth hormone receptors are single-chain transmembrane proteins; PRLr is composed of three separate domains: an extracellular region with 5 cysteines, which contains the prolactin binding site, a single transmembrane domain, and a cytoplasmic region, the length of which appears to influence ligand binding and regulate cellular function. Prolactin modulates immune system function by stimulating the proliferation and survival of cells through its receptor, present on T and B lymphocytes and macrophages. The function of PRL is mediated by a variety of signaling cascades as well as by the wide variety of PRLr isoforms observed in nature. A long form and several other isoforms are expressed in human tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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