SLC7A11 Antibody (711589) in WB

Western blot analysis was performed on membrane enriched extracts and tissue extracts (30 ug lysate) of SH-SY5Y (Lane 1), HeLa (Lane 2), Hep G2 (Lane 3), K-562 (Lane 4), Mouse Stomach (Lane 5) and U-87 MG (Lane 6). The blots were probed with ABfinity? Anti- xCT/ SLC7A11 Rabbit Recombinant Oligoclonal Antibody (Product ## 711589, 1-2 ug/ml) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal? Secondary Antibody, HRP conjugate (Product ## A27036, 0.4 ug/ml, 1:2500 dilution). A 55 kDa band corresponding to xCT/ SLC7A11 was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris gel (Product ## NP0321BOX), XCell SureLock? Electrophoresis System (Product ## EI0002) and Novex® Sharp Pre-Stained Protein Standard  (Product ## LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane by iBlot® Dry Blotting System (Product ## IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product ## WP20005).

Western blot analysis was performed on membrane enriched extracts and tissue extracts (30 ug lysate) of SH-SY5Y (Lane 1), HeLa (Lane 2), Hep G2 (Lane 3), K-562 (Lane 4), Mouse Stomach (Lane 5) and U-87 MG (Lane 6). The blots were probed with ABfinity? Anti- xCT/ SLC7A11 Rabbit Recombinant Oligoclonal Antibody (Product ## 711589, 1-2 ug/ml) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal? Secondary Antibody, HRP conjugate (Product ## A27036, 0.4 ug/ml, 1:2500 dilution). A 55 kDa band corresponding to xCT/ SLC7A11 was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris gel (Product ## NP0321BOX), XCell SureLock? Electrophoresis System (Product ## EI0002) and Novex® Sharp Pre-Stained Protein Standard  (Product ## LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane by iBlot® Dry Blotting System (Product ## IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product ## WP20005).

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