NFkB p65 Antibody (33-9900) in ChIP

ChIP- qPCR analysis of NF-kappa-B-p65 was performed with 3 µg/ml of the NF-kappa-B-p65 mouse monoclonal Antibody (339900) on sheared chromatin from 2 million HeLa cells treated with TNF-a (50ng/ml for 1h) using the MAGnify? Chromatin Immunoprecipitation System ( 49-2024). Normal mouse IgG (3µg/ml) was used as a negative IP control. The purified DNA from each ChIP sample was analyzed by StepOnePlus? Real-Time PCR System (4376600) with primers for the promoter of active IL-8 and IL-6 gene, used as positive control targets, and the GAPDH gene , used as negative control target. Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.

ChIP- qPCR analysis of NF-kappa-B-p65 was performed with 3 µg/ml of the NF-kappa-B-p65 mouse monoclonal Antibody (339900) on sheared chromatin from 2 million HeLa cells treated with TNF-a (50ng/ml for 1h) using the MAGnify? Chromatin Immunoprecipitation System ( 49-2024). Normal mouse IgG (3µg/ml) was used as a negative IP control. The purified DNA from each ChIP sample was analyzed by StepOnePlus? Real-Time PCR System (4376600) with primers for the promoter of active IL-8 and IL-6 gene, used as positive control targets, and the GAPDH gene , used as negative control target. Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.

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