Dual EGC Solution for Complex Carbohydrates | Thermo Fisher Scientific

Simplify the complexity for carbohydrate analysis

Automatically generate eluent gradients to analyze from simple to complex carbohydrates by operating your Thermo Scientific Dionex ICS-6000 HPIC system in Dual Eluent Generation Cartridge (Dual EGC) mode. The EGC cartridges in series with each other to generate the extremely reproducible and accurate KOH/KMSA eluent gradient to resolve mono, di- and oligosaccharides, as well as sialic acids.

The Dual EGC mode is a powerful Thermo Scientific Dionex "Just Add Water" electrolytic eluent generation technology. Making manual eluent preparation unnecessary, electrolytic eluent generation is a key feature of our Reagent-Free Ion Chromatography (RFIC) systems equipped with Thermo Scientific Chromeleon CDS software.

The 1 mm format Thermo Scientific CarboPac columns for Dual EGC provide predictable, high-resolution separations of monosaccharides, disaccharides, sialic acids, and complex oligosaccharides.

The columns are:

Browse our complete portfolio below, or let one of our experts help you tailor a selection specific to your application and workflow needs.

How to simplify carbohydrate analysis with Dual Eluent Generation and HPAE-PAD? Just add water and 1mm format CarboPac columns.

Related Products For Automated Carbohydrate Analysis

See how much you could save using Dual EGC Mode

The Dual EGC Mode Cost Savings Calculator helps you estimate your yearly savings when running your Thermo Scientific Dionex ICS-6000 HPIC system in Dual EGC mode rather than preparing eluents manually.

Why use Dual EGC for carbohydrate analysis?

Dual EGC for carbohydrate analysis provides faster, easier start-up, cleaner eluents, less maintenance, and greater reproducibility.

Eliminates manual preparation of eluents	Automated electrolytic eluent generation, reagent-free Eliminates variation inherent to manual eluent preparation, related to CO²
Improves IC reproducibility	High quality, accurate eluent concentrations every time Consistent and reproducible retention time with baseline stability
Maximizes instrument uptime, minimizes maintenance	Pump only use deionized water therefore minimizes maintenance and downtime Extends lifetime for pump seals and pistons as no acids or bases are in contact to precipitate
Offers eluent gradient capabilities	Only isocratic pump needed with RFIC to run gradient methods Easy and flexible to modify the eluent concentration with Thermo Scientific Chromeleon Chromatography Data System (CDS)
Eliminates sodium acetate issues	"Just-add-water" to analyze complex carbohydrate without sodium hydroxide/sodium acetate gradients Eliminates risk of contamination from lower quality sodium acetate

Ion chromatography system configuration in Dual EGC mode

Dual EGC mode is available on the Dionex ICS-6000 HPIC system to support complex carbohydrate analysis. In Dual EGC mode, this RFIC-enabled system employs methanesulfonic acid (MSA) and potassium hydroxide (KOH) EGC cartridges, in series, to electrolytically generate potassium hydroxide/potassium methanesulfonate (KOH/KMSA) eluents. This operating mode is applicable to analytical (1.0mm) column formats.

For analytical (1mm format) operation — The Thermo Scientific Dionex EGC 400 (KOH and MSA) Eluent Generator Cartridges are designed to enable operation at flow rates between 20µL/min–200µL/min. The Dionex EGC 400 MSA cartridge is connected in series to a Dionex EGC 400 KOH cartridge to generate KOH/KMSA eluents up to a total concentration of 200mM at flow rates of 20–63µL/min, and up to 63mM at flow rates up to 200µL/min.

See the difference in your oligosaccharide results

Data comparison: manual eluent preparation vs. Dual EGC mode eluent preparation (1mm format)

Click image to enlarge

Learn more

Reagent-Free Ion Chromatography with Eluent Generation (RFIC-EG)

This informational web page provides answers to:

Analyzing Carbohydrates by HPAE-PAD

This informational web page:

Discusses how high-performance anion-exchange chromatography with pulsed amperometric detection (HPAE-PAD) can simplify carbohydrate analysis
Provides links to major application notes for HPAE-PAD carbohydrate analysis

Resources

Application - The product is For General Lab Use Only - Not For Diagnostic Procedures

Our General Purpose product-lines are not intended for in vitro diagnostic purposes in accordance with our product documentation, manuals, and labels. They are designated for General Laboratory Use Only.

Our Products have not been tested or validated for such applications and their use for in vitro diagnostic purposes may result in inaccurate results and, more seriously, health and safety risks.

The product is For General Lab Use Only - Not For Diagnostic Procedures. The application is For Research Use Only. Not for use in diagnostic procedures.

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Dual EGC Solution for Carbohydrate Analysis