Thermo Scientific™

Dionex™ CarboPac™ PA1 IC 1 mm Columns for Dual EGC Applications

Catalog number: 303272
Thermo Scientific™

Dionex™ CarboPac™ PA1 IC 1 mm Columns for Dual EGC Applications

Catalog number: 303272

For predictable high resolution separation of mono-, di-, and some oligosaccharides, use the Thermo Scientific™ Dionex™ CarboPac™ PA1 (1 mm) columns, when operating in Dual EGC mode on Thermo Scientific™ Dionex™ ICS-6000 HPIC™ systems equipped with Thermo Scientific™ Chromeleon™ CDS software. Dual EGC mode enables electrolytic generation of potassium methane sulfonate and potassium hydroxide gradients (KMSA/KOH) gradient eluent for carbohydrate analysis using a Dionex KOH EGC 400 KOH Eluent Generator Cartridge and a Dionex MSA EGC 400 MSA Eluent Generator Cartridge in series. Using the Dual EGC allows for an expanded set of samples for gradient separations of some oligosaccharides along with mono-, di-, and trisaccharide's using electrolytically generated potassium methane sulfonate and potassium hydroxide gradients with a combined concentration up to 200 mM. Many separations require only an isocratic separation. However, some groups of analytes will require a step or gradient elution. Retention of carbohydrates can be varied with eluent concentration, in some cases changing the elution order as the eluent concentration increases.

 
Catalog Number
303272
Unit Size
Each
Type
Separator Column
Anion Exchange Capacity
6.25 µeq/column
Length (Metric)
250 mm
Price (USD)
Full specifications
Column Capacity6.25 µeq
For Use With (Application)Dual EGC Applications
For Use With (Equipment)Thermo Scientific Dionex ICS-6000 HPIC Systems Equipped with Thermo Scientific Chromeleon CDS Software
Max. Pressure4500 psi (310 bar) at 0.063 mL/min.
Stationary PhasePolystyrene 2% Cross-linked with Divinylbenzene
TypeSeparator Column
pH0 to 14
Length (Metric)250 mm
Diameter (Metric)1 mm
Temperature (Metric)4°C to 60°C
Column ConstructionPEEK
Anion Exchange Capacity6.25 µeq/column
Resin Composition10 μm diameter substrate (polystyrene 2% cross-linked with divinylbenzene) agglomerated with 500 nm microbead quaternary ammonium functionalized latex (5% cross-linked)
Unit SizeEach
Catalog Number
Unit Size
Each
Type
Guard Column
Anion Exchange Capacity
1.25 µeq/column
Length (Metric)
50 mm
Price (USD)
Full specifications
Column Capacity1.25 µeq
For Use With (Application)Dual EGC Applications
For Use With (Equipment)Dionex CarboPac PA1, 1 x 250 mm Analytical Column
Max. Pressure4500 psi (310 bar) at 0.063 mL/min.
Stationary PhasePolystyrene 2% Cross-linked with Divinylbenzene
TypeGuard Column
pH0 to 14
Length (Metric)50 mm
Diameter (Metric)1 mm
Temperature (Metric)4°C to 60°C
Column ConstructionPEEK
Anion Exchange Capacity1.25 µeq/column
Resin Composition10 μm diameter substrate (polystyrene 2% cross-linked with divinylbenzene) agglomerated with 500 nm microbead quaternary ammonium functionalized latex (5% cross-linked)
Unit SizeEach
Showing 2 of 2
Catalog NumberSpecificationsUnit SizeTypeAnion Exchange CapacityLength (Metric)Price (USD)
303272Full specifications
EachSeparator Column6.25 µeq/column250 mmRequest A Quote
Column Capacity6.25 µeq
For Use With (Application)Dual EGC Applications
For Use With (Equipment)Thermo Scientific Dionex ICS-6000 HPIC Systems Equipped with Thermo Scientific Chromeleon CDS Software
Max. Pressure4500 psi (310 bar) at 0.063 mL/min.
Stationary PhasePolystyrene 2% Cross-linked with Divinylbenzene
TypeSeparator Column
pH0 to 14
Length (Metric)250 mm
Diameter (Metric)1 mm
Temperature (Metric)4°C to 60°C
Column ConstructionPEEK
Anion Exchange Capacity6.25 µeq/column
Resin Composition10 μm diameter substrate (polystyrene 2% cross-linked with divinylbenzene) agglomerated with 500 nm microbead quaternary ammonium functionalized latex (5% cross-linked)
Unit SizeEach
303273Full specifications
EachGuard Column1.25 µeq/column50 mmRequest A Quote
Column Capacity1.25 µeq
For Use With (Application)Dual EGC Applications
For Use With (Equipment)Dionex CarboPac PA1, 1 x 250 mm Analytical Column
Max. Pressure4500 psi (310 bar) at 0.063 mL/min.
Stationary PhasePolystyrene 2% Cross-linked with Divinylbenzene
TypeGuard Column
pH0 to 14
Length (Metric)50 mm
Diameter (Metric)1 mm
Temperature (Metric)4°C to 60°C
Column ConstructionPEEK
Anion Exchange Capacity1.25 µeq/column
Resin Composition10 μm diameter substrate (polystyrene 2% cross-linked with divinylbenzene) agglomerated with 500 nm microbead quaternary ammonium functionalized latex (5% cross-linked)
Unit SizeEach
Showing 2 of 2

Combined with pulsed amperometric detection (PAD), this anion-exchange column provides fast, reproducible separations with no need for derivatization. The 1 mm (standard/microbore) Dionex CarboPac PA1, 1 mm columns for Dual EGC contain the same resin as the proven 4 mm and 2 mm Dionex CarboPac PA1 columns, which are used for non-Dual EGC carbohydrate analysis.

Unique advantages of Dionex CarboPac PA1 Columns for Dual EGC

  • Predictable, high-resolution, separation of monosaccharide and disaccharides using electrolytically generated potassium methanesulfonate and potassium hydroxide
  • Rugged all-purpose column to determine monosaccharides and disaccharides in a variety of matrices
  • Column chemistry approved for use in a variety of official methods for the analysis of foods (AOAC Methods 995.13, 996.04, 997.08, 2000.11, 2000.17 and 2001.02)

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Frequently asked questions (FAQs)

Citations & References