For predictable high resolution separation of mono-, di-, and some oligosaccharides, use the Thermo Scientific™ Dionex™ CarboPac™ PA1 (1 mm) columns, when operating in Dual EGC mode on Thermo Scientific™ Dionex™ ICS-6000 HPIC™ systems equipped with Thermo Scientific™ Chromeleon™ CDS software. Dual EGC mode enables electrolytic generation of potassium methane sulfonate and potassium hydroxide gradients (KMSA/KOH) gradient eluent for carbohydrate analysis using a Dionex KOH EGC 400 KOH Eluent Generator Cartridge and a Dionex MSA EGC 400 MSA Eluent Generator Cartridge in series. Using the Dual EGC allows for an expanded set of samples for gradient separations of some oligosaccharides along with mono-, di-, and trisaccharide's using electrolytically generated potassium methane sulfonate and potassium hydroxide gradients with a combined concentration up to 200 mM. Many separations require only an isocratic separation. However, some groups of analytes will require a step or gradient elution. Retention of carbohydrates can be varied with eluent concentration, in some cases changing the elution order as the eluent concentration increases.
| Column Capacity | 6.25 µeq |
| For Use With (Application) | Dual EGC Applications |
| For Use With (Equipment) | Thermo Scientific Dionex ICS-6000 HPIC Systems Equipped with Thermo Scientific Chromeleon CDS Software |
| Max. Pressure | 4500 psi (310 bar) at 0.063 mL/min. |
| Stationary Phase | Polystyrene 2% Cross-linked with Divinylbenzene |
| Type | Separator Column |
| pH | 0 to 14 |
| Length (Metric) | 250 mm |
| Diameter (Metric) | 1 mm |
| Temperature (Metric) | 4°C to 60°C |
| Column Construction | PEEK |
| Anion Exchange Capacity | 6.25 µeq/column |
| Resin Composition | 10 μm diameter substrate (polystyrene 2% cross-linked with divinylbenzene) agglomerated with 500 nm microbead quaternary ammonium functionalized latex (5% cross-linked) |
| Unit Size | Each |
| Column Capacity | 1.25 µeq |
| For Use With (Application) | Dual EGC Applications |
| For Use With (Equipment) | Dionex CarboPac PA1, 1 x 250 mm Analytical Column |
| Max. Pressure | 4500 psi (310 bar) at 0.063 mL/min. |
| Stationary Phase | Polystyrene 2% Cross-linked with Divinylbenzene |
| Type | Guard Column |
| pH | 0 to 14 |
| Length (Metric) | 50 mm |
| Diameter (Metric) | 1 mm |
| Temperature (Metric) | 4°C to 60°C |
| Column Construction | PEEK |
| Anion Exchange Capacity | 1.25 µeq/column |
| Resin Composition | 10 μm diameter substrate (polystyrene 2% cross-linked with divinylbenzene) agglomerated with 500 nm microbead quaternary ammonium functionalized latex (5% cross-linked) |
| Unit Size | Each |
| Catalog Number | Specifications | Unit Size | Type | Anion Exchange Capacity | Length (Metric) | Price (USD) | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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| 303272 | Each | Separator Column | 6.25 µeq/column | 250 mm | Request A Quote | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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| 303273 | Each | Guard Column | 1.25 µeq/column | 50 mm | Request A Quote | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Combined with pulsed amperometric detection (PAD), this anion-exchange column provides fast, reproducible separations with no need for derivatization. The 1 mm (standard/microbore) Dionex CarboPac PA1, 1 mm columns for Dual EGC contain the same resin as the proven 4 mm and 2 mm Dionex CarboPac PA1 columns, which are used for non-Dual EGC carbohydrate analysis.
Unique advantages of Dionex CarboPac PA1 Columns for Dual EGC
