Validation of HLA Typing by NGS

Presenter: Eric T. Weimer, Ph.D. (ABHI) | Director of the Molecular Immunology laboratory, Associate Director of the Flow Cytometry, HLA, and Microbiology laboratories, and Assistant Professor of Pathology and Laboratory Medicine at the University of North Carolina at Chapel Hill

Dr. Weimer presents a real-world example from the University of North Carolina Hospital to showcase an HLA Laboratory's experience when validating a new technology in Next Generation Sequencing. The presentation includes a summary of NGS validation procedures, in-depth analysis of quality control measures, as well as pre-implementation cost analysis and preparation of the ASHI submission packet for NGS typing accreditation. 

Topics discussed:

  • Review of UNC's Approach to NGS Validation
  • Validation Summary and Quality Control Analysis
  • Validation Considerations
  • NGS Cost Analysis
  • Preparation of ASHI Accreditation Packet