Super AquaBlue ELISA Substrate, 500 mL - FAQs

TMB is a faster developing, stronger substrate that yields greater amplification/sensitivity/background. The use of an acid stop solution produces a yellow end product read at 450nm. Super AquaBlue is a slower developing blue/green substrate particularly useful for kinetic studies.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

When working with our matched pairs or uncoated ELISA kits, please refer to the user guide provided with your antibody-matched pair for kit-specific guidance. When designing your own ELISA, please utilize our ELISA Builder tool for reagent selection as well as our ELISA Development and Optimization pages for futher guidance.

Find additional tips, troubleshooting help, and resources within our Antibodies and Immunoassays Support Center.