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ThPOK Monoclonal Antibody (2POK), PE, eBioscience™
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FIGURE: 1 / 1
ThPOK Antibody (12-5928-82) in Flow
C57Bl/6 thymocytes were stained with Anti-Mouse CD4 APC (Product # 17-0041-82) and Anti-Mouse CD8a FITC (Product # 11-0081-82), followed by intracellular staining with 0.125 µg of Rat IgG2a K Isotype Control PE (Product # 12-4321-80) (blue histogram) or 0.125 µg of Anti-Mouse ThPOK PE using the Foxp3 Staining Buffer Set and protocol (Product # 00-5523-00). The histogram demonstrates ThPOK staining of CD4 SP (purple histogram) and CD8 SP (orange histogram) thymocytes.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
12-5928-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rat
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
2POK
Conjugate
PE
PE
PE
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: This 2POK monoclonal antibody reacts with mouse ThPOK, which is also known as c-Krox and Zbtb7b. In hematopoietic cells, this 78 kDa transcription factor is expressed in thymocytes and T lymphocytes in the spleen and lymph nodes. In non-hematopoietic cells, ThPOK can be detected in the skin, heart, smooth muscle, and liver. Studies demonstrate that ThPOK promotes CD4+ lineage differentiation by inhibiting the expression of CD8 lineage-specific genes such as Runx3. ThPOK has also been shown to inhibit Cd4 silencing, another key facet required for differentiation to the CD4+ lineage. In addition to its role in thymocyte development, ThPOK is involved in the effector functions of invariant natural killer T (iNKT) cells and gamma delta T cells.
Applications Reported: This 2POK antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This 2POK antibody has been tested by intracellular staining and flow cytometric analysis of mouse thymocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
ZFP67 is an early growth response gene that encodes a zinc finger-containing transcription factor that binds to the promoter regions of type I collagen genes (e.g., COL1A1; MIM 120150) and has a role in development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DKFZp686G01254; krueppel-related zinc finger protein cKrox; RP11-307C12.9; T-helper-inducing POZ/Krueppel-like factor; unnamed protein product; Vzinc finger and BTB domain containing 7B; zinc finger protein 67; zinc finger protein Th-POK
Gene Aliases: c-Krox; Thpok; Zfp67
UniProt ID: (Mouse) Q64321
Entrez Gene ID: (Mouse) 22724
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
core promoter proximal region sequence-specific DNA binding
bacterial-type RNA polymerase transcriptional repressor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
identical protein binding
protein homodimerization activity
histone deacetylase binding
sequence-specific double-stranded DNA binding
negative regulation of transcription from RNA polymerase II promoter
NK T cell differentiation
regulation of transcription from RNA polymerase II promoter
lactation
regulation of gene expression
positive regulation of gene expression
negative regulation of gene expression
positive regulation of interleukin-17 production
response to insulin
regulation of CD4-positive, alpha-beta T cell differentiation
positive regulation of CD4-positive, alpha-beta T cell differentiation
regulation of CD8-positive, alpha-beta T cell differentiation
negative regulation of CD8-positive, alpha-beta T cell differentiation
positive regulation of fat cell differentiation
positive regulation of transcription from RNA polymerase II promoter
positive regulation of insulin receptor signaling pathway
negative regulation of NK T cell proliferation
positive regulation of brown fat cell differentiation
positive regulation of cold-induced thermogenesis
adaptive thermogenesis
negative regulation of T-helper 17 cell differentiation
positive regulation of SREBP signaling pathway
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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