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IL-2 Monoclonal Antibody (JES6-5H4), PE, eBioscience™
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FIGURE: 1 / 23
IL-2 Antibody (12-7021-41) in Flow
Nature communications 2024 -
Longevity of CTH522 specific immunity and protection. a Experimental outline. B6C3F1 mice were immunized with 10 µg CTH522/CAF(r)01 or Sham-immunized. b Kinetics of CTH522 specific IgG: week 8, 31, 56 n = 20 (except CTH522 week 56 n = 18), week 59 and 61, n = 6, NT 50: CTH522 week 8, 31, 56 n = 19 (except week 56 n = 17), Controls n = 20, week 59 and 61, n = 6 and IFN-gamma responses: n = 6 (except Controls... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
12-7021-41
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Human,
Mouse
Host/Isotype
Rat
/ IgG2b, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
JES6-5H4
Conjugate
PE
PE
PE
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The JES6-5H4 antibody reacts with mouse interleukin-2 (IL-2), a 17 kDa T cell growth factor and a major immunoregulatory cytokine.
Applications Reported: JES6-5H4 has been reported for use in intracellular flow cytometric analysis.
Applications Tested: This JES6-5H4 antibody has been pre-titrated and tested ny intracellular staining of 6-hour PMA and Ionomycin-activated mouse splenocytes. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: H-IL-2; il 2; IL2; ILN; Interleukin; interleukin 2 prepeptide; interleukin-2; Interleukin2; Involved in regulation of T cell clonal expansion; M-IL-2; T-cell growth factor; T-cell growth factor (TCGF); TCGF
Gene Aliases: Il-2
UniProt ID: (Mouse) P04351
Entrez Gene ID: (Mouse) 16183
negative regulation of protein phosphorylation
positive regulation of protein phosphorylation
leukocyte activation involved in immune response
positive regulation of immunoglobulin production
negative regulation of B cell apoptotic process
immune response
G-protein coupled receptor signaling pathway
phospholipase C-activating G-protein coupled receptor signaling pathway
positive regulation of cytosolic calcium ion concentration
gene expression
positive regulation of B cell proliferation
positive regulation of interferon-gamma production
positive regulation of interleukin-17 production
interleukin-2-mediated signaling pathway
positive regulation of T cell proliferation
positive regulation of activated T cell proliferation
positive regulation of tyrosine phosphorylation of STAT protein
response to ethanol
positive regulation of T cell differentiation
positive regulation of regulatory T cell differentiation
positive regulation of transcription from RNA polymerase II promoter
regulation of T cell homeostatic proliferation
positive regulation of isotype switching to IgG isotypes
negative regulation of lymphocyte proliferation
negative regulation of inflammatory response
positive regulation of dendritic spine development
extrinsic apoptotic signaling pathway in absence of ligand
receptor signaling pathway via STAT
positive regulation of plasma cell differentiation
negative regulation of T-helper 17 cell differentiation
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