Mouse splenocytes were unstimulated (left) or stimulated with PMA/Ionomycin in the presence of Brefeldin A (right) overnight, and then surface stained with Anti-Mouse CD4 APC (Product # 17-0041-82), followed by intracellular staining with 0.06 µg of Anti-Mouse IL-2 PerCP-Cyanine5-5. Cells in the lymphocyte gate were used for analysis.

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IL-2 Antibody — Intracellular staining of stimulated mouse splenocytes. As expected based on known expression patterns, IL-2 clone JES6-5H4 stains a large subset of CD4+ T cells and a minor subset of NK1.1+ NK cells with no staining observed without stimulation. Details: Mouse splenocytes were cultured in the presence of Protein Transport Inhibitors (500X) (Unstimulated, bottom row) or Cell Stimulation Cocktail (plus protein transport inhibitors, 500X) for 5 hours (Stimulated, top row). Cells were fixed and permeabilized with the IC Fixation & Permeabilization Buffer Set and protocol followed by intracellular staining with CD4 (clone RM4-5), NK1.1 (clone PK136) and IL-2 (clone JES6-5H4). Cells in the CD4+ (blue histogram) or NK1.1+ (purple histogram) gates were used for analysis. {TM}

Product Details

45-7021-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.125 µg/test

View 21 publications 21 publications

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG2b, kappa

Recommended Isotype Control

Rat IgG2b kappa Isotype Control (eB149/10H5), PerCP-Cyanine5.5, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

JES6-5H4

Conjugate

PerCP-Cyanine5.5 PerCP-Cyanine5.5 PerCP-Cyanine5.5

Excitation/Emission Max

489/679 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

100 µg

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_925755

Product Specific Information

Description: The JES6-5H4 antibody reacts with mouse interleukin-2 (IL-2), a 17 kDa T cell growth factor and a major immunoregulatory cytokine.

Applications Reported: This JES6-5H4 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This JES6-5H4 antibody has been tested by intracellular staining and flow cytometric analysis of stimulated mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 695 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: H-IL-2; il 2; IL-2; ILN; Interleukin; Interleukin-2; Interleukin2; Involved in regulation of T cell clonal expansion; M-IL-2; T-cell growth factor; T-cell growth factor (TCGF); TCGF

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Gene Aliases: Il-2; Il2

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UniProt ID: (Mouse) P04351

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Entrez Gene ID: (Mouse) 16183

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Function(s)

cytokine activity interleukin-2 receptor binding growth factor activity carbohydrate binding kappa-type opioid receptor binding glycosphingolipid binding actin or actin-binding cytoskeletal protein non-receptor serine/threonine protein kinase

Process(es)

Performance Guarantee

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IL-2 Monoclonal Antibody (JES6-5H4), PerCP-Cyanine5.5, eBioscience™

IL-2 Antibody (45-7021-82) in Flow

Product Details

45-7021-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Amount

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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