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Invitrogen
IL-2 Monoclonal Antibody (JES6-5H4), Alexa Fluor™ 488, eBioscience™
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Product Details
53-7021-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Human,
Mouse
Host/Isotype
Rat
/ IgG2b, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
JES6-5H4
Conjugate
Excitation/Emission Max
499/520 nm
View spectra
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The JES6-5H4 antibody reacts with mouse interleukin-2 (IL-2), a 17 kDa T cell growth factor and a major immunoregulatory cytokine.
Applications Reported: The JES6-5H4 antibody has been reported useful for ELISA, ELISPOT, neutralization, and intracellular staining for flow cytometric analysis.
Applications Tested: The Alexa Fluor® 488 JES6-5H4 antibody has been tested by intracellular staining and flow cytometric analysis and can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: H-IL-2; il 2; IL-2; ILN; Interleukin; Interleukin-2; Interleukin2; Involved in regulation of T cell clonal expansion; M-IL-2; T-cell growth factor; T-cell growth factor (TCGF); TCGF
Gene Aliases: Il-2; Il2
UniProt ID: (Mouse) P04351
Entrez Gene ID: (Mouse) 16183
negative regulation of protein phosphorylation
positive regulation of protein phosphorylation
immune system process
negative regulation of B cell apoptotic process
immune response
G-protein coupled receptor signaling pathway
positive regulation of cytosolic calcium ion concentration
protein kinase C-activating G-protein coupled receptor signaling pathway
positive regulation of B cell proliferation
positive regulation of interferon-gamma production
positive regulation of interleukin-17 production
positive regulation of T cell proliferation
positive regulation of activated T cell proliferation
positive regulation of tyrosine phosphorylation of Stat5 protein
positive regulation of T cell differentiation
positive regulation of regulatory T cell differentiation
negative regulation of heart contraction
positive regulation of transcription from RNA polymerase II promoter
regulation of T cell homeostatic proliferation
positive regulation of isotype switching to IgG isotypes
negative regulation of lymphocyte proliferation
negative regulation of inflammatory response
positive regulation of immunoglobulin secretion
positive regulation of dendritic spine development
extrinsic apoptotic signaling pathway in absence of ligand
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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