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Perforin Monoclonal Antibody (dG9 (delta G9)), PE, eBioscience™
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FIGURE: 1 / 8
Perforin Antibody (12-9994-41) in Flow
Staining of normal human peripheral blood cells with Anti-Human CD8 APC-eFluor® 780 (Product # 47-0088-42) followed by fixation and permeabilization with the Foxp3 Staining Buffers (Product # 00-5523-00) and intracellular staining with Mouse IgG2b K Isotype Control PE (Product # 12-4732-81) (left) or Anti-Human Perforin PE (right). Cells in the lymphocyte gate were used for analysis.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
12-9994-41
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Pig
Published species
Human,
Pig
Host/Isotype
Mouse
/ IgG2b, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
dG9 (delta G9)
Conjugate
PE
PE
PE
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The dG9 antibody clone reacts with human perforin (pore-forming protein, pfp). Perforin is one of the cytolytic mediators present in the cytoplasmic granules of cytotoxic T lymphocytes (CTL) and natural killer cells (NK). Perforin is involved in the killing function by CTLs and NKs and has an important role in the immune response against tumors and virus infections.
Applications Reported: This dG9 (delta G9) antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This dG9 (delta G9) antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Perforin is one of the major cytolytic proteins of cytolytic granules. Perforin is a cytolytic mediator and is stored in and released by cytoplasmic granules. Moreover, perforin is involved in immune defense against tumors and virus infections as mediated by cytotoxic lymphocytes. Perforin is a 555 amino acid protein with a 21 amino acid signal peptide, and has a molecular weight of 70 to 75 kD. Perforin is a pore forming protein with a mechanism of transmembrane channel formation similar to C9, and homology between perforin and C9 have been demonstrated. Studies show that perforin is expressed only in killer cell lines and not in helper T lymphocytes or other tumor cells tested. Perforin is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in the perforin gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. Alternative splicing results in multiple transcript variants of perforin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cytolysin; lymphocyte pore-forming protein; OMAK; OTTHUMP00000019759; perforin 1 (pore forming protein); Perforin1; PGFL; PIGF; PIGF-2; PLGF; pore forming protein; pore-forming protein; PRF1 (pore forming protein 1); RP11-710A11.3; unnamed protein product
Gene Aliases: HPLH2; P1; PFP
UniProt ID: (Human) P14222
Entrez Gene ID: (Human) 5551, (Pig) 396595
immunological synapse formation
leukocyte mediated cytotoxicity
T cell mediated cytotoxicity
defense response to tumor cell
immune system process
immune response to tumor cell
apoptotic process
defense response
cellular defense response
protein secretion
protein import
protein homooligomerization
defense response to virus
positive regulation of killing of cells of other organism
protein transmembrane transport
granzyme-mediated programmed cell death signaling pathway
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