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Invitrogen
CD4 Monoclonal Antibody (OKT4 (OKT-4)), Functional Grade, eBioscience™
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Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
16-0048-85
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Hamster,
Human
Host/Isotype
Mouse
/ IgG2b, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
OKT4 (OKT-4)
Conjugate
Functional Grade
Functional Grade
Functional Grade
Form
Liquid
Concentration
1 mg/mL
Amount
500 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The OKT4 monoclonal antibody reacts with human CD4, a 59 kDa cell surface glycoprotein expressed by the majority of thymocytes, a subpopulation of mature T cells (T-helper cells) and in low levels on monocytes. CD4 is a receptor for the human immunodeficiency virus (HIV). The OKT4 antibody recognizes a different epitope than the RPA-T4 monoclonal antibody, and these antibodies do not cross-block binding to each other's respective epitopes.
Applications Reported: This OKT4 (OKT-4) antibody has been reported for use in flow cytometric analysis and immunoprecipitation.
Applications Tested: This OKT4 (OKT-4) antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Storage and handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: Greater than 90%, as determined by SDS-PAGE.
Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.
Aggregation: Less than 10%, as determined by HPLC.
Target Information
The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD4 antigen (p55); CD4 antigen p55; CD4 receptor; cd4a; fCD4; Leu-3; T-cell surface antigen T4/Leu-3; T4 surface glycoprotein precursor; unnamed protein product
Gene Aliases: CD4mut; IMD79; Leu-3; OKT4D; T4
UniProt ID: (Human) P01730
Entrez Gene ID: (Human) 920
virus receptor activity
transmembrane signaling receptor activity
receptor binding
extracellular matrix structural constituent
protein binding
zinc ion binding
lipid binding
coreceptor activity
immunoglobulin binding
enzyme binding
protein kinase binding
MHC class II protein complex binding
signaling receptor activity
interleukin-16 binding
interleukin-16 receptor activity
MHC class II protein binding
identical protein binding
protein homodimerization activity
protein tyrosine kinase binding
positive regulation of immune system process
immune response
cell adhesion
signal transduction
cell surface receptor signaling pathway
enzyme linked receptor protein signaling pathway
transmembrane receptor protein tyrosine kinase signaling pathway
positive regulation of calcium ion transport into cytosol
T cell differentiation
macrophage differentiation
leukocyte chemotaxis
response to estradiol
maintenance of protein location in cell
positive regulation of interleukin-2 production
response to vitamin D
helper T cell enhancement of adaptive immune response
interleukin-15-mediated signaling pathway
positive regulation of T cell proliferation
T cell activation
T cell selection
response to ethanol
positive regulation of monocyte differentiation
viral entry into host cell
positive regulation of inflammatory response
T cell receptor signaling pathway
regulation of T cell activation
positive regulation of multicellular organismal process
cellular response to granulocyte macrophage colony-stimulating factor stimulus
response to methamphetamine hydrochloride
cellular response to ionomycin
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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