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Invitrogen

IL-4 Monoclonal Antibody (11B11), PE-Cyanine7, eBioscience™

41 Published Figures
43 References
View all (96) IL-4 antibodies

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Product Details

25-7041-80

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

0.25 µg/test
View 43 publications 43 publications
Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG1, kappa

Recommended Isotype Control

Rat IgG1 kappa Isotype Control (eBRG1), PE-Cyanine7, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

11B11

Conjugate

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

Excitation/Emission Max

569/780 nm View spectra spectra

Form

Liquid

Concentration

0.2 mg/mL

Amount

25 µg

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2573519

Product Specific Information

Description: The 11B11 antibody reacts with mouse interleukin-4 (IL-4), a 14 kD cytokine. The 11B11 antibody is a neutralizing antibody. mouse IL-4, also known as BCDF, BCGF-1 and BSF-1, is secreted by activated Th2 and natural killer T cells, and to a lesser extent by Th1 and mast cells. IL-4 is species-specific and promotes proliferation and differentiation of B cells. IL-4, in conjunction with other signals such as CD40, induces Ig isotype switching of B cells and the upregulation of surface MHC class II and CD23.

Applications Reported: This 11B11 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This 11B11 antibody has been tested by intracellular staining and flow cytometric analysis of stimulated mouse splenocytes using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to BestProtocols®: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This antibody can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Bioinformatics

Protein Aliases: B-cell growth factor 1; B-cell IgG differentiation factor; B-cell stimulatory factor 1; H-IL-4; IGG1 induction factor; IL4; ILN; Interleukin; interleukin mRNA; Interleukin4; lymphocyte stimulatory factor 1; M-IL-4; MGC79402; unnamed protein product

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Gene Aliases: BSF-1; Il-4

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UniProt ID: (Mouse) P07750

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Entrez Gene ID: (Mouse) 16189

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Function(s)
cytokine activity cytokine receptor binding interleukin-4 receptor binding growth factor activity
Process(es)
negative regulation of transcription from RNA polymerase II promoter microglial cell activation positive regulation of protein phosphorylation innate immune response in mucosa positive regulation of defense response to virus by host T-helper 1 cell lineage commitment positive regulation of immunoglobulin production negative regulation of acute inflammatory response negative regulation of chronic inflammatory response positive regulation of leukocyte chemotaxis negative regulation of leukocyte activation immune response JAK-STAT cascade cholesterol metabolic process positive regulation of cell proliferation response to nematode regulation of proton transport response to selenium ion positive regulation of gene expression negative regulation of epithelial cell migration positive regulation of macroautophagy positive regulation of cell migration positive regulation of B cell proliferation B cell costimulation negative regulation of tumor necrosis factor production positive regulation of chemokine production positive regulation of interleukin-10 production positive regulation of interleukin-13 production interleukin-4-mediated signaling pathway positive regulation of T cell proliferation positive regulation of activated T cell proliferation T cell activation macrophage activation positive regulation of tyrosine phosphorylation of STAT protein defense response to protozoan myeloid dendritic cell differentiation negative regulation of macrophage activation positive regulation of mast cell degranulation negative regulation of nitric oxide biosynthetic process T-helper 2 cell differentiation leukocyte activation positive regulation of MHC class II biosynthetic process positive regulation of T cell differentiation negative regulation of osteoclast differentiation negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of receptor-mediated endocytosis positive regulation of isotype switching to IgE isotypes positive regulation of isotype switching to IgG isotypes negative regulation of inflammatory response positive regulation of peptidyl-tyrosine phosphorylation regulation of immune response negative regulation of T cell activation positive regulation of B cell activation negative regulation of white fat cell proliferation positive regulation of mononuclear cell migration dendritic cell differentiation extrinsic apoptotic signaling pathway in absence of ligand positive regulation of cold-induced thermogenesis neuroinflammatory response positive regulation of beta-amyloid clearance positive regulation of myoblast fusion positive regulation of cellular respiration positive regulation of reactive oxygen species biosynthetic process negative regulation of complement-dependent cytotoxicity negative regulation of cellular response to transforming growth factor beta stimulus negative regulation of T-helper 17 cell differentiation negative regulation of endothelial cell apoptotic process positive regulation of eosinophil chemotaxis positive regulation of B cell chemotaxis positive regulation of T-helper 2 cell cytokine production positive regulation of ATP biosynthetic process negative regulation of extrinsic apoptotic signaling pathway
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