This product is manufactured in our Grand Island, US facility and is intended for sale in North America, Latin America, and the Asia/Pacific region—higher freight charges will apply to orders shipped elsewhere.

Trypsin (1:250), powder

Catalog number:  27250018

Related applications:

Mammalian Cell Culture

Back to top


Gibco® Trypsin 1:250 powder is an irradiated mixture of proteases derived from porcine pancreas. Due to its digestive strength it is widely used for cell dissociation, during routine cell culture passaging, and primary tissue dissociation. We offer a variety of Gibco® Trypsin and animal origin free TrypLE™ that feature:

Quality Testing
Documented Traceability
Dual-site cGMP Manufacturing

Quality Testing
Gibco® Trypsin 1:250 is tested for endotoxin, mycoplasma, bacterial, fungal and viral contamination. Other quality tests include multiple activity assays, ash analysis and moisture analysis.

Documented Traceability
We can provide detailed documentation to meet your regulatory needs. Gibco® Trypsin information available includes lot traceability, animal origin certificates, lot analyses, irradiation certificates, a viral inactivation summary, and supply chain transparency.

cGMP Manufacturing and Quality System
For supply chain continuity, we repack Gibco® trypsin at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements, are certified to ISO 13485, and are registered with the FDA as medical device manufacturers.
For Research Use or Further Manufacturing. Not for use in diagnostic procedures.


Chelators: No EDTA
Culture Type: Adherent Cell Culture
Endotoxin Level: Low
Mass: 100 g
Product Line: Gibco®
Reagent Type: Trypsin
Shipping Condition: Room Temperature
Tested For: In Vitro Bioassay
Form: Powder
Phenol Red Indicator: No Phenol Red
Concentration: 250 X
Format: Securitainer Capped Vial

Contents & storage

Storage conditions: 2–8°C.
Shipping conditions: Ambient.
Shelf life: 24 months from date of manufacture.