ABC Peroxidase Standard Staining Kit

Catalog number: 32020

Thermo Scientific™  Related applications: Cellular Imaging

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Description

Thermo Scientific Pierce ABC Staining Kits include reagents for the avidin-biotin complex (ABC) technique, a highly sensitive method for immunohistochemical detection with biotinylated secondary antibodies.

Pierce ABC Staining Kits are highly sensitive, produce very low background staining and have rapid avidin-biotin interactions. Standard Kits contain only the two essential reagents (avidin and biotinylated HRP) can be used with nearly any species of biotinylated secondary antibody. The Ultra-Sensitive ABC Peroxidase Staining Kits are approximately five times more sensitive than the regular-senstivity kit, enabling more dilute primary antibodies to be used while producing equal staining intensity. Ultra-Sensitive Kits are offered as a Standard Kit and as Complete Kits, which contain biotinylated secondary antibodies and appropriate block serum for use with mouse or rabbit antibodies, respectively.

Related Products
Ultra-Sensitive ABC Peroxidase Standard Staining Kit
Ultra-Sensitive ABC Peroxidase Mouse IgG Staining Kit
Ultra-Sensitive ABC Peroxidase Rabbit IgG Staining Kit
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Technique: IHC (Immunohistochemistry), Immunodetection
Product Size: 4 mL
Substrate Type: HRP (Horseradish Peroxidase) Substrate
Detection Method: Colorimetric, Chromogenic, Biotin-Based

Contents & storage

Sufficient For: At least 10 mL of ABC staining solution
• Reagent A (Avidin), 2 mL
• Reagent B (Biotinylated HRP), 2 mL
• Mixing bottle with dropper dispenser tip, 1

Store all reagents at 4°C

Documents

Manuals & protocols